Skip to main content
SpringerLink
Log in

The preparation of xenoantiserum specific for a murine mastocytoma cell line

  • Original Articles
  • Published:
Cancer Immunology, Immunotherapy Aims and scope Submit manuscript

Summary

A method is described whereby a xenoantiserum directed toward membrane components of DBA/2 murine mastocytoma P815 cells was raised. This antiserum was found to be specific for tumor cell extracts and had no reactivity with comparable extracts of normal cells when tested by complement fixation. The antiserum was capable of killing P815 cells in the presence of guinea-pig complement but had no reactivity with L1210 leukemia cells or a variety of normal DBA/2 cell preparations. When mixed with varying numbers of tumor cells and injected IP to either DBA/2 or B6D2 Fl mice, the antiserum demonstrated a protective effect by either prolonging survival time or, when low numbers of cells were injected, apparently facilitating complete removal from the body of tumor cells. When administered following resection of SC grown tumors in B6D2 Fl mice, the antiserum prevented tumor recurrence in 50% of treated mice in comparison to animals treated with normal rabbit serum, in which recurrence occurred in all test animals.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Billing, R. J., Safani, M., Peterson, P.: Isolation and characterization of human B cell alloantigens. J. Immunol. 117, 1589 (1976)

    Google Scholar 

  • Gold, P., Freedman, S. O.: Demonstration of tumour-specific antigens in human colonic carcinomata by immunological tolerance and absorption techniques. J. Exp. Med. 121, 439 (1965)

    Google Scholar 

  • Greaves, M. F.: Clinical applications of cell surface markers. Prog. Hematol. 4, 255 (1975)

    Google Scholar 

  • Kelly, B., Levy, J. G.: Evidence for a common tumour-associated antigen in extracts of human bronchogenic carcinoma. Br. J. Cancer 35, 828 (1977)

    Google Scholar 

  • Möller, J.: In: Immunological tolerance and immune responses by lymphocytes: their nature and regulation. Landy, M., Braun, W. (eds.), p. 215. London: Academic Press 1969

    Google Scholar 

  • Takei, F., Levy, J. G., Kilburn, D. G.: In vitro induction of cytotoxicity against syngeneic mastocytoma and its suppression by spleen and thymus cells from tumour-bearing mice. J. Immunol. 116, 288 (1976)

    Google Scholar 

  • Weiner, R. S., Hubbard, J. D., Mardiney, M. R.: Production of tumor-specific antibody in the xenogenic host. J. Natl. Cancer Inst. 49, 1063 (1972)

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Microbiology, University of British Columbia, V6T 1W5, Vancouver, British Columbia, Canada

    A. Al-Rammahy & Julia G. Levy

Authors
  1. A. Al-Rammahy
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Julia G. Levy
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Al-Rammahy, A., Levy, J.G. The preparation of xenoantiserum specific for a murine mastocytoma cell line. Cancer Immunol Immunother 6, 163–167 (1979). https://doi.org/10.1007/BF00205541

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00205541

Keywords

Search

Navigation