Summary
The majority of lymphocytes separated from tumor cell suspensions were T cells. Conjugates of T lymphocytes and tumor cells were often seen. Variable numbers of T cells exhibited signs of activation such as the ability to form stable E rosettes and attachment to normal and malignant cells (a phenomenon designated natural attachment: NA). A proportion of T cells activated in vitro by allogeneic stimulation regularly exhibit these properties. The T cell-tumor conjugates in the suspensions may represent the NA phenomenon, but they could also be the product of T cells that adhere on the basis of specific recognition of cell surface antigens.
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Abbreviations
- BBS:
-
balanced salt solution
- E rosettes:
-
rosettes formed with sheep erythrocytes
- EA rosettes:
-
rosettes formed with ox erythrocytes coated with anti-ox IgG
- FCS:
-
fetal calf serum
- MLC:
-
mixed lymphocyte cultures
- NA:
-
natural attachment
- PBL:
-
peripheral blood lymphocytes
- SRBC:
-
sheep erythrocytes
- T lymphocytes:
-
thymusderived lymphocytes
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Galili, U., Vanky, F., Rodriguez, L. et al. Activated T lymphocytes within human solid tumors. Cancer Immunol Immunother 6, 129–133 (1979). https://doi.org/10.1007/BF00205536
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DOI: https://doi.org/10.1007/BF00205536