Summary
The present study investigated the role of antigen-presenting cells (APC) in the activation of noncytolytic T cells against tumor antigens. The noncytolytic-type T cells exerted their antitumor effect by producing γ-interferon (IFN-γ) and by activating macrophages as the ultimate effectors. The production of IFN-γ by these noncytolytic T cells following the stimulation with tumor cells required the participation of Ia+ APC, since the depletion of APC from cultures of tumor-immunized spleen cells resulted in almost complete inhibition of the IFN-γ production. Both L3T4+ and Lyt-2+ subsets of T cells were capable of producing IFN-γ, and the requirement of APC for the production of IFN-γ was the case irrespective of whether noncytolytic T cells were of L3T4+ or Lyt-2+ phenotype. More importantly, it was demonstrated that the production of IFN-γ by L3T4+ and Lyt-2+ T cells was inhibited by addition of the respective anti-class II and anti-class I H-2 antibody to cultures. These results indicate that antitumor L3T4+ or Lyt-2+ noncytolytic T cells are activated for the IFN-γ production by recognizing tumor antigens in the context of self-class II or -class I H-2 molecules on APC.
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This work was supported by a Grant-in-Aid for the Special Project Cancer-Bioscience from the Ministry of Education, Science and Culture, Japan
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Sakamoto, K., Nakajima, H., Shimizu, J. et al. The mode of recognition of tumor antigens by noncytolytic-type antitumor T cells: Role of antigen-presenting cells and their surface class I and class II H-2 molecules. Cancer Immunol Immunother 27, 261–266 (1988). https://doi.org/10.1007/BF00205449
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DOI: https://doi.org/10.1007/BF00205449