Summary
Samples of complete human semen were incubated in gastric juice for different time periods at 37°C, and by simulating the post-mortem temperature decrease of the human body. The changes in the spermatozoa were similar in both experiments. Short incubation specimens were examined directly with interference contrast microscopy and showed an almost immediate immobilization of spermatozoa when they were brought in contact with gastric juice. Specimens with longer incubation periods were stained with alcalic fuchsine and examined by immersion microscopy. There was a morphologically stable plateau for the heads of the spermatozoa for up to 6h of incubation. The tails disappeared progressively in the first 45 min. After more than 6h of incubation a progressive swelling and lysis of the heads was observed. Spermatozoa could be recognized for up to 7 days of incubation.
Zusammenfassung
Menschliche Spermaproben wurden bei 37°C für unterschiedliche Zeitspannen mit Magensaft versetzt und die Änderungen von Motilität und Morphologie der Spermien untersucht. In einer zweiten Versuchsanordnung wurde zusätzlich die postmortale Temperatursenkung simuliert. In beiden Versuchsanordnungen waren die Spermienbefunde identisch. Die Spermien wurden nahezu unmittelbar nach Kontakt mit dem Magensaft immobilisiert. Bei den längeren Inkubationszeiten verschwanden alle Spermienschwänze in den ersten 45 min, die Morphologie der Spermienköpfe änderte sich nicht während der ersten 6h, danach kam es schnell zu einer Lysis. Dennoch konnten bis zum 7. Inkubationstag morphologisch normale Spermien beobachtet werden.
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Hooft, P., van de Voorde, H. In vitro changes in human spermatozoa exposed to gastric juice: Laboratory findings as a support for forensic practice. Z Rechtsmed 101, 41–44 (1988). https://doi.org/10.1007/BF00205323
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DOI: https://doi.org/10.1007/BF00205323