Summary
Simple, rapid methods are described for G3m(21) typing with peroxidase-labeled monoclonal anti-G3m(21) antibody. In G3m(21) typing by ELISA, microtiter wells were coated directly with the test antigen, which was detected with the enzyme-labeled monoclonal antibody. To further simplify the procedure, a dot immunobinding method was developed. The antigen in the test serum applied onto a nitrocellulose membrane was successfully detected with the enzyme-labeled monoclonal antibody. These methods, particularly the dot immunobinding, are suitable for forensic casework because they are rapid and simple and require no technical skill.
Zusammenfassung
Es werden einfache schnelle Methoden zur G3m(21)-Typisierung mit enzymisch markierten monoklonalen Anti-G3m(21)-Antikörper beschrieben. Bei G3m(21)-Typisierung mit ELISA wurden Mikrotitertüpfel mit zu untersuchendem Antigen direkt überzogen. Das Antigen wurde mit dem enzymisch markierten monoklonalen Antikörper nachgewiesen. Eine Punkt-Immunobindung Methode wurde geschaffen, um das Prozedere noch weiter vereinfachen zu können. Das Antigen in der Probe wurde auf einer Nitrozellulosemembran appliziert und mit dem enzymisch markierten monoklonalen Antikörper erfolgreich nachgewiesen. Diese Methoden, besonders die Punkt-Immunobindung, sind für die forensische Praktik geeignet.
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References
Fletcher SM, Dorrill MJ, Dolton P (1984) Bloodstain allotyping: An ELISA method for G1m(3), Dev Biol Stand 57:381–384
Francois-Gerard C, Hoste B (1984) A double-sandwich ELISA technique for Gm typing with a mouse monoclonal antibody. 18th Congress of the International Society of Blood Transfusion, Munich, July, 22–27, 1984
Galfre G, Howe SC, Milstein C, Butcher GW, Howard JC (1977) Antibodies to major histocompatibility antigens produced by hybrid cell lines. Nature 266:550–552
Grubb R (1956) Agglutination of erythrocytes coated with “incomplete” anti-Rh by certain rheumatoid arthritic sera and some other sera. Acta Pathol Microbiol Scand 39:195–197
Jefferis R, Reimer CB, Skvaril F, de Lange G, Ling NR, Lowe J, Walker MR, Phillips DJ, Aloisio CH, Wells TW, Vaerman JP, Magnusson CG, Kubagawa H, Cooper M, Vartdal F, Vandvik B, Haaijman JJ, Makela O, Sarnesto A, Lando Z, Gergely J, Rajnavölgyi E, László G, Radl J, Molinaro GA (1985) Evaluation of monoclonal antibodies having specificity for human IgG sub-classes: Results of an IUIS/WHO collaborative study. Immunol Lett 10:223–252
Kishida T, Tamaki Y (1983) An improved method for the production of antisera to G1m allotypes. Jpn J Hum Genet 28:269–272
Kishida T, Tamaki Y (1984) Gm typing by enzyme-linked immunosorbent assay (ELISA). Z Rechtsmed 93:23–27
Nakane PK, Kawaoi A (1974) Peroxidase-labeled antibody. A new method of conjugation. J Histochem Cytochem 22:1084–1091
Tamaki Y, Kishida T, Shibata K, Takahashi N, Fukuda M (1981) Preparation of anti-Gm sera by immunization of rabbits with protein A-fractionated normal IgG proteins: Further study. J Immunol Methods 45:177–182
Tamaki Y, Takahashi N, Kishida T, Ishikawa K (1984) Production of monoclonal anti-G3m(21) antibody. Jpn J Legal Med 38:152–154
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Kishida, T., Tamaki, Y. & Fukuda, M. G3m(21) typing by ELISA and dot immunobinding with enzyme-labeled monoclonal anti-G3m(21) antibody. Z Rechtsmed 101, 15–20 (1988). https://doi.org/10.1007/BF00205319
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DOI: https://doi.org/10.1007/BF00205319