Summary
Batches of BCG to be used as a scarification vaccine by suspension in a new liquid may be preserved at 4, −25, and −70° C. During storage the colony-forming units have been found to be practically unchanged after 3 months at 4° C, 9 months at −25° C, and 3 years at −70° C. The biological controls in animals agree with those in vitro.
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References
Berthrong, M.: The macrophage tubercle bacillus relationship and resistance to tuberculosis Ann. New York Acad. Sci. 154, 157 (1968)
Chambon, L.: Etudes sur la préparation d'un vaccin B.C.G. lyophilisé thermostable pour usage intradermique. Rapport sur le fonctionnement technique de l' Institut Pasteur de Dakar 58, 1965 (1971)
Edwards, L. B., Palmer, C. E., Magnus, K.: La vaccination par le B.C.G. Influence de la température et de la durée de conservation du vaccin B.C.G. O.M.S.: Série de Monographies 12, 71 (1954)
Gheorghiu, M.: Principes théoriques actuels de la lyophilisation. Microbiologia, Epidemiologia, Parasitologia 4, 363 (1968)
Gheorghiu, M., Kosloff, F., Paris, C.: Contrôle de la qualité du vaccin B.C.G. lyophilisé par inoculation à la souris International Symposium on B.C.G. vaccine Frankfurt/Main. Series Immunobiol. Standard 17, 221 (1971)
Gheorghiu, M., Kosloff, F., Derudder, J.: Etude de la thermostabilité du vaccin. B.C.G. intradermique lyophilisé (souche de l'Institut Pasteur). Progress in Immunobiol. Standard 5, 437 (1972)
Grover, A. A., Kim, H. K., Wiegeshaus, E. H., Smith, D. W.: I. Hast parasite relationship in experimental airborne tuberculosis. II. Reproducible infection by means of an inoculum preserved at −70° C. Journal of Bacteriology 832 (1967)
Kim, T. H., Kubica, G. P.: Long-term preservation and storage of Mycobacteria. Applied Microbiology 311 (1972)
Lind, A.: Stability of dried B.C.G. vaccine stored at −70° C, −25° C and 4° C. Scand. J. resp. Dis. 48, 343 (1967)
Mackaness, G. B., Smith, N., Wells, A. Q.: The growth of intracellular tubercle bacilli in relation to their virulence. Amer. Re. Tuberc. 69, 479 (1954)
Mackaness, G. B., Auclair, D. J., Lagrange, P. H.: Immunopotentialisation with B.C.G. — Immune response to different strains and preparations. Journal of the National Cancer Inst. 51, 1655 (1973)
Mathé, G., Halle-Pannenko, O., Bourut, C.: B.C.G. in cancer immunotherapy: results obtaines with various B.C.G. preparations in a screening study for systemic adjuvants applicable to cancer immunoprophylaxis or immunotherapy. Nat. Cancer. Inst. Monograph. 39, 107 (1973)
Obayashi, Y.: Le vaccin B.C.G. desséché. O.M.S. 142 (1957) (1957)
Rostand, J.: Glycérine et la résistance du sperme aux basses temperatures. Compte-rendu 222, 1524 (1946)
Rostand, J.: Sur le refroidissement des cellules spermatiques en présence de glycérine. Compte-rendu 234, 2310 (1952)
Smith, A. U., Polge, C.: Survival of spermatozoa at low temperatures. Nature 166, 668 (1950)
Toida, I.: Nad-ase of tuberculous guinea pigs. Amer. Rev. resp. Dis. 94, 625 (1966)
Ungar, J., Stanic, M.: The effect of DMSO on B.C.G. freezedried vaccine. International Symposium on B.C.G. vaccine Frankfurt/Main. Series Immunobiol. Standard 17, 179 (1971)
W.H.O./TB: Techn. Guide, 67.6
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Gheorghiu, M., Chambon, L. Preservation of fresh BCG scarification vaccine at 4, −25, and −70° C. Cancer Immunol Immunother 1, 11–16 (1976). https://doi.org/10.1007/BF00205288
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DOI: https://doi.org/10.1007/BF00205288