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Retention of immunogenicity after X-irradiation of mouse colon tumor cells expressing the transfected influenza virus hemagglutinin gene

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Summary

We have previously demonstrated that murine colon tumor cells transfected with the gene coding for the hemagglutination antigen (HA) of influenza virus acquire an inherent immunogenicity, fail to grow in syngeneic mice, and demonstrate an ability to cross-protect against a challenge with parental nontransfected cells. In the present study the immunogenic potential of HA-transfected cells correlated with cell-surface HA expression, as measured both by a fluorescence-activated cell sorter and by radiolabeled antibody binding. HA-transfected immunogenic cells had a median lethal dose (LD50) that was 10000-fold greater than that of nontransfected cells. Most importantly this study demonstrated that HA-transfected cells retained their immunogenicity after X-irradiation with 12000 rad. This characteristic makes their potential usefulness in treating human neoplasia more plausible.

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References

  1. Boon T (1983) Antigenic tumor cell variants obtained with mutagens. Adv Cancer Res 39:121

    Google Scholar 

  2. Boone CW, Blackman K (1972) Augmented immunogenicity of tumor cell homogenates infected with influenza virus. Cancer Res 32:1018

    Google Scholar 

  3. Brattain MG, Strobel-Stevens J, Find D, Webb M, Sarrif AM (1980) Establishment of mouse colonic carcinoma cell lines with different metastatic properties. Cancer Res 40:2142

    Google Scholar 

  4. Fearon ER, Itaya T, Hunt B, Vogelstein B, Frost P (1988) The induction of immunogenic tumor variants by transfection with a foreign gene. Cancer Res 38:2975

    Google Scholar 

  5. Frost P, Kerbel R, Bauer E, Tartamella-Biondo R, Cefalu W (1983) Mutagen treatment as a means for selecting immunogenic variants from otherwise poorly immunogenic malignant murine tumors. Cancer Res 43:125

    Google Scholar 

  6. Hamaoka T, Fujiwara H, Teshima K, Aoki H, Yamamoto H, Kitagawa M (1979) Regulatory function of hapten-reactive helper and suppressor T lymphocytes. III. Amplification of generation of tumor-specific cytotoxic effector T lymphocytes by suppressor T cell-depleted hapten reactive T lymphocytes. J Exp Med 149:185

    Google Scholar 

  7. Hosokawa M, Okayasu T, Ikeda K, Katoh H, Susuki H, Kobayashi I (1983) Alteration of immunogenicity of xenogenized tumor cells in syngeneic rats by the immune response to virus-associated antigens produced on immunizing cells. Cancer Res 43:2301

    Google Scholar 

  8. Itaya T, Yamagiwa S, Okada AF, Oikawa T, Kuzamaki N, Takeichi N, Hosokawa M, Kobayashi H (1987) Xenogenization of a mouse lung carcinoma (3LL) by transfection with an allogeneic class 1 major histocompatibility complex gene (h-2Ld). Cancer Res 47:3136

    Google Scholar 

  9. Kerbel RS, Frost P, Carlow DA, Elliott BE (1984) Possible epigenetic mechanisms of tumor progression: induction of high-frequency heritable but phenotypically unstable changes in the tumorigenic and metastatic properties of tumor cell populations by 5-azacytidine treatment. J Cell Physiol Suppl 3:87

    Google Scholar 

  10. Kobayashi H, Takeichi N, Kuzumaki N (1978) Xenogenization of tumor cells in xenogenization of lymphocytes, erythroblasts, and tumor cells. Hokkaido Univ Med Libr Ser 10:117

    Google Scholar 

  11. Lachman PJ, Sikora K (1978) Coupling PPD to tumor cells enhances their antigenicity in BCG-primed mice. Nature 271:463

    Google Scholar 

  12. Lindenmann J, Klein PA (1967) Increased immunogenicity of host cell antigen associated with influenza virus. J Exp Med 126:93

    Google Scholar 

  13. Reed L, Muench H (1938) A simple method of estimating fifty percent endpoints. Am J Hyg 27:493

    Google Scholar 

  14. Sambrook J, Rogers L, White J, Gething MJ (1985) Lines of BPV-transformed mouse cells that constitutively express influenza virus hemagglutinin. EMBO J 4:91

    Google Scholar 

  15. Sella A, Hunt B, Frost P (1989) Mutagen induced Imm+ variants: The need for viable and cloned Imm+ variants for effective protection against a primary murine tumor and its metastases. Clin Exp Metastasis 7:97–105

    Google Scholar 

  16. Yamaguchi H, Moriuchi T, Hosokawa M, Kobayashi H (1982) Increased or decreased immunogenicity of tumor-associated antigen according to the amount of virus-associated antigen in rat tumor cells infected with Friend virus. Cancer Immunol Immunother 12:119

    Google Scholar 

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Authors and Affiliations

  1. Department of Cell Biology, The University of Texas M. D. Anderson Cancer Center, 77030, Houston, Texas

    Toshiyuki Itaya, Barbara Hunt & Philip Frost

  2. Department of Medicine, The University of Texas M. D. Anderson Cancer Center, 77030, Houston, Texas

    Philip Frost

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  1. Toshiyuki Itaya
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  2. Barbara Hunt
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  3. Philip Frost
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Itaya, T., Hunt, B. & Frost, P. Retention of immunogenicity after X-irradiation of mouse colon tumor cells expressing the transfected influenza virus hemagglutinin gene. Cancer Immunol Immunother 28, 248–252 (1989). https://doi.org/10.1007/BF00205233

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  • DOI: https://doi.org/10.1007/BF00205233

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