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A simple assay for fluorescent siderophores produced by Pseudomonas species and an efficient isolation of pseudobactin

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Abstract

Several iron binding metabolites (siderophores) of Pseudomonas fluorescens B10 (JL-3133) have been detected using C18 reverse phase HPLC coupled with photodiode array detection methods. This analysis utilized a volatile mobile phase of 90% 20 mm NH4HCO3/10% MeOH, pH 6.5. It has been shown to be applicable to other P. fluorescens strains for the detection of related metabolites. Direct scale-up of the analytical HPLC conditions allowed for the efficient preparative isolation of pseudobactin, the principle siderophore produced by P. fluorescens B10 (JL-3133).

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References

  • Abdallah MA. 1991 Pyoverdins and pseudobactins. In: Winkelmann G, ed. CRC Handbook of Microbial Iron Chelates. Boca Raton, FL: CRC Press; 139–153.

    Google Scholar 

  • Budzikiewicz H. 1988 Peptide Siderophores from Pseudomonas. Nat Prod Chem 3, 317–333.

    Google Scholar 

  • Buyer JS, Leong J. 1986 Iron transport-mediated antagonism between plant growth-promoting and plant-deleterious Pseudomonas Strains. J Biol Chem 261, 791–794.

    Google Scholar 

  • Buyer JS, Wright JM, Leong J. 1986 Structure of pseudobactin A214, a siderophore from a bean-deleterious Pseudomonas. Biochemistry 25, 5492–5499.

    Google Scholar 

  • Demange P, Wendenbaum S, Bateman A, Dell A, Abdallah MA. 1987 Bacterial siderophores: structure and physiochemical properties of pyoverdins and related compounds. In: Winkelmann G, van der Helm D, Neilands JB, eds. Iron Transport in Microbes, Plants, and Animals. London: VCH.

    Google Scholar 

  • Demange P, Bateman A, Dell A, Abdallah MA. 1988 Structure of Azotobactin D, a siderophore of Azotobacter vinelandii Strain D (CCM 289). Biochemistry 27, 2745–2752.

    Google Scholar 

  • Demange P, Wendenbaum S, Linget C, et al. 1990 Bacteriol siderophores: structure and NMR assignment of pyoverdins Pa, siderophore of Pseudomonas aeruginosa ATCC 15692. Biol Met 3, 155–170.

    Google Scholar 

  • King EO, Ward MK, Raney DE. 1954 Two simple media for the demonstration of pyocyanin and fluorescin. J Lab Clin Med 44, 301–307.

    CAS  PubMed  Google Scholar 

  • Kloepper JW, Leong J, Teintze M, Schroth MN. 1980 Enhanced plant growth by siderophores produced by plant growth-promoting rhizobacteria. Nature 286, 885–886.

    Google Scholar 

  • Kloepper JW, Schroth MN. 1981a Plant growth-promoting rhizobacteria and plant growth under gnotobiotic conditions. Phytopathology 71, 642–644.

    Google Scholar 

  • Kloepper JW, Schroth MN. 1981b Relationship of in vitro antibiosis of plant growth-promoting rhizobacteria to plant growth and the displacement of root microflora. Phytopathology 71, 1020–1024.

    Google Scholar 

  • Konetschny-Rapp S, Huschka HG, Winkelmann G, Jung G. 1988 High performance liquid chromatography of siderophore from fungi. Biol Met 1, 9–17.

    Google Scholar 

  • Konetschny-Rapp S, Jung G, Raymond K, Meiwes J, Zähner H. 1992 Solution thermodynamics of the ferric complexes of new desferrioxamine siderophores obtained by directed fermentation. J Am Chem Soc 114, 2224–2230.

    Google Scholar 

  • Loper J, Lindow SE. 1987 Lack of evidence for in situ fluorescens pigment production by P. syringae pv. syringae on bean leaf surfaces. Phytopathology 77, 1449–1454.

    Google Scholar 

  • Meyer JM, Abdallah MA. 1978 The fluorescent pigment of Pseudomonas fluorescens: biosynthesis, purification, and physiochemical properties. J Gen Microbiol 107, 319–328.

    CAS  Google Scholar 

  • Mishaghi IJ, Olsen MW, Cotty PJ, Donndelinger CR. 1988 Fluorescent siderophore mediated iron deprivation —a contingent biological control mechanism. Soil Biol Biochem 20, 573–574.

    Google Scholar 

  • Persmark M, Frejd T, Mattiasson B. 1990 Purification, characterization, and structure of pseudobactin 589A, a siderophore from a plant growth promoting Pseudomonas. Biochemistry 29, 7348–7356.

    Google Scholar 

  • Philson SB, Llinás M. 1982 Siderochromes from Pseudomonas fluorescens. J Biol Chem 257, 8081–8085.

    Google Scholar 

  • Stainer RY, Palleroni NJ, Doudoroff M. 1966 The aerobic pseudomonads: a taxonomic study. J Gen Microbiol 43, 159–271.

    CAS  PubMed  Google Scholar 

  • Teintze M, Hossain MB, Barnes CL, Leong J, van der Helm D. 1981 Stucture of ferric pseudobactin, a siderophore from a plant growth promoting Pseudomonas. Biochemistry 20, 6446–6457.

    Google Scholar 

  • Teintze M, Leong J. 1981 Structure of ferric pseudobactin A, a second siderophore from plant growth promoting Pseudomonas B10. Biochemistry 20, 6457–6462.

    Google Scholar 

  • Yang CC, Leong J. 1984 Structure of pseudobactin 7SR1, a siderophore from a plant-deleterious Pseudomonas. Biochemistry 23, 3534–3540.

    Google Scholar 

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  1. Department of Chemistry, Oregon State University, Corvallis, OR, USA

    Brian Nowak-Thompson & Steven J. Gould

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  1. Brian Nowak-Thompson
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  2. Steven J. Gould
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Nowak-Thompson, B., Gould, S.J. A simple assay for fluorescent siderophores produced by Pseudomonas species and an efficient isolation of pseudobactin. Biometals 7, 20–24 (1994). https://doi.org/10.1007/BF00205189

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  • DOI: https://doi.org/10.1007/BF00205189

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