Abstract
This paper presents a hydroponic system for culturing and maintaining the VAM fungus Glomus intraradices in symbiosis with linseed (Linum usitatissimum L.) under greenhouse conditions in pure nutrient solution. It was possible to obtain large quantities of mycorrhizal host plant roots as well as extramatrical mycelium and chlamydospores free of impeding residues of solid substrate components. Starting from linseed donor plants inoculated in sand and transferred to the nutrient solution, new infections arose within the fast growing root system, hyphae spread out into the liquid and infected mycorrhiza-free receptor plants. Data for infection rates and plant growth parameters are presented. In comparsion to other culture systems for VAM fungi, the advantages of this hydroponic system are discussed and potential uses suggested.
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References
Balestrini R, Berta G, Bonfante P (1992) The plant nucleus in mycorrhizal roots: positional and structural modifications. Biol Cell 75:235–243
Dehne H W, Backhaus GF (1986) The use of vesicular-arbuscular mycorrhizal fungi in plant production. I. Inoculum production. Z Pflanzenkr Pflanzenschutz 93:415–424
Elmes RP, Mosse B (1984) Vesicular-arbuscular endomycorrhizal inoculum production. II. Experiments with maize (Zea mays) and other hosts in nutrient flow culture. Can J Bot 62:1531–1536
Janardhanan KK, Gupta M L, Husain A (1990) Axenic culture of a vesicular-arbuscular mycorrhizal fungus. Curr Sci 59:509–513
Khan AG (1993) Occurrence and importance of mycorrhizae in aquatic trees of New South Wales, Australia. Mycorrhiza 3:31–38
Lei J, Bécard G, Catford JG, Piché Y (1991) Root factors stimulate 32P uptake and plasmalemma ATPase activity in the vesicular-arbuscular mycorrhizal fungus Gigaspora margarita. New Phytol 118:289–294
Michelsen A (1993) The mycorrhizal status of vascular epiphytes in Bale Mountains National Park, Ethiopia. Mycorrhiza 4:11–15
Millner PD, Kitt DG (1992) The Beltsville method for soilless production of vesicular-arbuscular mycorrhiza fungi. Mycorrhiza 2:9–15
Mosse B, Thompson J P (1984) Vesicular-arbuscular endomycorrhizal inoculum production. I. Exploratory experiments with beans (Phaseolus vulgaris) in nutrient flow culture. Can J Bot 62:1523–1530
Ojala JC, Jarrell WM (1980) Hydroponic sand culture system for mycorrhizal research. Plant Soil 57:297–303
Palma JM, Longa MA, Río LA del, Arines J (1993) Superoxide dismutase in vesicular-arbuscular mycorrhizal red clover plants. Physiol Plant 87:77–83
Peuss H (1958) Untersuchungen zur Ökologie und Bedeutung der Tabakmycorrhiza. Arch Microbiol 29:112–142
Schönbeck F, Grunewaldt-Stöcker G, Alten H von (1994) Mycorrhizae. In: Campbell CC, Benson DM (eds) Epidemiology and management of root diseases. Springer, Berlin Heidelberg New York (in press)
Stenlund DL, Charvat ID (1994) Vesicular-arbuscular mycorrhizae in floating wetland mat communities dominated by Typha. Mycorrhiza 4:131–137
Sylvia DM, Hubbel DH (1986) Growth and sporulation of vesicular-arbuscular mycorrhizal fungi in aeroponic and membrane systems. Symbiosis 1:259–267
Urbach W, Rupp W, Sturm H (1983) Praktikum zur Stoffwechselphysiologie der Pflanzen. Thieme, Stuttgart New York
Vilariño A, Arines J, Schüepp H (1993) Extraction of vesicular-arbuscular mycorrhizal mycelium from sand samples. Soil Biol Biochem 25:99–100
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Dugassa, D.G., Grunewaldt-Stöcker, G. & Schönbeck, F. Growth of Glomus intraradices and its effect on linseed (Linum usitatissimum L.) in hydroponic culture. Mycorrhiza 5, 279–282 (1995). https://doi.org/10.1007/BF00204962
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DOI: https://doi.org/10.1007/BF00204962