Summary
We have used multicolor fluoresence in situ hybridization of banded chromosomes to orient FcγRII and clone 1054 on a single early metaphase chromosome band (1q22) representing about 2% of the physical map of chromosome 1 in the Charcot-Marie-Tooth (CMT1B) gene region. These two cloned fragments are on the same partially digested 900-kb MluI fragment detected by pulsed field gel electrophoresis. When applied to data from an earlier study, multicolor in situ hybridization results further refined the CMT1B genetic location from an 18 cM interval to a 6 cM interval and the physical map from 15% of chromosome 1 to 3% of chromosome 1. Occasionally the three FcγRII immunoglobulin receptor genes within the 200-kb region are resolved in individual metaphase chromatids.
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References
Albertson DG (1985) Mapping muscle protein genes by in situ hybridization using biotin-labeled probes. EMBO J 4:2493–2498
Anderson LA, Hall JM, Lebo RV, King MC (1989) New polymorphism on chromosome 1q near PUM. (10th International Workshop on Human Gene Mapping) Cytogenet Cell Genet 51:951
Brown-Shimer S, Johnson KA, Lawrence JB, Johnson C, Bruokin A, Green NR, Hill DE (1990) Molecular cloning and chromosome mapping of the human gene encoding protein phosphotyrosyl phosphatase 1B. Proc Natl Acad sci USA 87:5148–5152
Donis-Keller H, Green P, Helms C, Cartinhour S, Weiffenbach B, Stephens K, Keith TP, Bowden DW, Smith DR, Lander ES, Botstein D, Akots B, Rediker KS, Gravius T, Brown VA, Rising MB, Parker C, Powers JA, Watt DE, Kauffman ER, Bricker A, Phipps P, Muller-Kahle H, Braman JC, Knowlton RG, Barker DF, Crooks SM, Lincoln SE, Daly MJ, Abrahamson J (1987) A genetik linkage map of the human genome. Cell 51: 319–337
Grundy HO, Pletz GA, Moore KW, Golbus MS, Jackson LG, Lebo RV (1989) The polymorphic Fcγ receptor II gene maps to human chromosome 1q. Immunogenetics 29:331–339
Harper ME, Saunders GF (1981) Localization of single-copy DNA sequences of G-banded human chromosomes by in situ hybridization. Chromosoma 83:431–439
Human Gene Mapping 8 (1985) 8th International Workshop on Human Gene Mapping. Cytogenet Cell Genet 40:67–106
Human Gene Mapping 10 (1989) 10th International Workshop on Human Gene Mapping. Cytogenet Cell Genet 51:67–90
Kingsmore SF, Watson ML, Howard TA, Seidin MF (1989) A 6000 kb segment of chromosome 1 is conserved in human and mouse. EMBO J 8:4073–4080
Landegenet JE, Jansen in de Wal N, Van Ommen GJB, Baas F, De Vijlder JJM, Van Duijn P, Ploeg M van der (1985) Chromosome localization of a unique gene by non-autoradiographic in situ hybridization. Nature 317:175–177
Langer-Safer PR, Levine M, Ward DC (1982) Immunological method for mapping genes on Drosophila polytene chromosomes. Proc Natl Acad sci USA 79:4381–4385
Latov N, Hays AP, Sherman WH (1988) Peripheral neuropathy and anti-MAG antibodies. CRC Crit Rev Neurobiol 3:301–332
Lawrence JB, Villnave CA, Singer RH (1988) Sensitive, high-resolution chromatin and chromosome mapping in situ: presence and orientation of two closely integrated copies of EBV in a lymphoma line. Cell 52:51–61
Lawrence JB, Singer RH, McNeil JA (1990) Interphase and metaphase resolution of different distances within the human dystrophin gene. Science 249:928–932
Lebo RV, Golbus MS, Cheung MC (1986) Detecting abnormal human chromosome constitutions by dual-laser flow cytogenetics. Am J Med Genet 25:519–529
Lebo RV, Anderson LA, DiMauro S, Lynch E, Hwang P, Fletterick R (1990) Rare McArdle disease locus polymorphic site on 11q13 contains CpG sequence. Hum Genet 86:17–24
Lebo RV, Lynch ED, Chance PF, O'Connell P, Leach R, Lalouel J-M, Golbus MS, Bird TD (1991a) Multicolor fluroescence in situ hybridization and linkage analysis cytogenetically order CMT1A gene region loci. Am J Hum Genet 47:A188 (in press)
Lebo RV, Chance PF, Dyck PJ, Redila-Flores MT, Lynch ED, Golbus MS, Bird TD, King MC, Anderson LA, Hall J, Wiegant J, Jiang Z, Dazin PF, Punnett HH, Schonberg SA, Moore K, Shull MM, Gendler S, Hurko O, Cornblath DR, Johns DR, Lovelace R, Latov N, Trofatter J, Conneally PM (1991b) Chromosome 1 Charcot-Marie-Tooth syndrome (CMT1B) locus in FcγRII gene region. Hum Genet 88:1–12
Lichter P, Tang CJC, Call K, Hermanson G, Evans GA, Housman D, Ward DC (1990) High-resolution mapping of human chromosome 11 by in situ hybridization with cosmid clones. Science 247:64–69
Lynch E, Ploeg M van der, Wiegant J, Moore K, Trounstine M, Lebo R (1990) Multicolor fluorescence in situ hybridization cytogenetically orders gene loci. Am J Hum Genet 47: A256
Nederlof PM, Robinson D, Abuknesha R, Wiegant J, Hopman AH, Tanke HJ, Raap AK (1989) Three-color fluorescence in situ hybridization for the simultaneous detection of multiple nucleic acid sequences. Cytometry 10:20–27
O'Connell P, Lathrop GM, Nakamura Y, Leppert ML, Ardinger RH, Murray JL, Lalouel J-M, White R (1989) Twenty-eight loci form a continuous linkage map of markers for human chromosome 1. Genomics 4:12–20
Olson MV, Dutchik JE, Graham MY (1986) Random-clone strategy for genomic restriction mapping in yeast. Proc Natl Acad Sci USA 83:7826–7830
Pinkel D, Landegent J, Collins C, Fuscoe J, Segraves R, Lucas J, Gray J (1988) Fluorescence in situ hybridization with human chromosome-specific libraries: detection of trisomy 21 and translocations of chromosome 4. Proc Natl Acad Sci USA 85: 9138–9142
Qiu WQ, Bruin D de, Brownstein BH, Pearse R, Ravetch JV (1990) Organization of the human and mouse low-affinity FcγR genes: duplication and recombination. Science 248:732–735
Raap AK, Hopman AHN, Ploeg M van der (1989) Hapten labeling of nucleic acid probes for DNA in situ hybridization. Tech Immunocytochem 4:167–197
Raeymaekers P, Van Broeckhoven C, Backhovens H, Wehnert A, Muylle L, De Jonghe P, Gheuens J, Vandenberghe A (1988) The Duffy blood groups is linked to the alpha-spectrin locus in a large pedigree with autosomal dominant inheritance of Charcot-Marie-Tooth disease type 1. Hum Genet 78:76–78
Rodriquez M, Lennon VA (1990) Immunoglobulins promote remyelination in the central nervous system. Ann Neurol 27:12–17
Schnedl W, Mikelsaar AV, Breitenbach M, Dann O (1977) DIPI and DAPI: fluorescence banding with only negligible fading. Hum Genet 36:167–172
Shaw PJ, Agard DA, Hiraoka Y, Sedat JW (1989) Tilted view reconstruction in optical microscopy: three-dimensional reconstruction of Drosophila melanogaster embryo nuclei. Biophys J 55:101–110
Shroyer KR, Nakane PK (1983) Use of DNP-labeled cDNA for in situ hybridization. J Cell Biol 97:337a
Shull MM, Pugh DG, Lingrel JB (1989) Characterization of human Na, K-ATPase α2 gene and identification of intragenic restriction fragment length polymorphisms. J Biol Chem 264: 17532–17543
Thomas FP, Lebo RV, Ding X-S, Lee SS, Latov N, Hayes AP (1991) Tomaculous neuropathy in chromosome 1 Charcot-Marie-Tooth syndrome (HMSN1B) Neurology 41:339
Trask B, Pinkel D, Engh G van den (1989) The proximity of DNA sequences in interphase cell nuclei is correlated to genomic distance and permits ordering of cosmids spanning 250 kilobase pairs. Genomics 5:710–717
Vedeler CA (1987) Demonstration of Fcγ receptors on human peripheral nerve fibres. J Neuroimmunol 15:207–216
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Lebo, R.V., Lynch, E.D., Wiegant, J. et al. Multicolor fluorescence in situ hybridization and pulsed field electrophoresis dissect CMT1B gene region. Hum Genet 88, 13–20 (1991). https://doi.org/10.1007/BF00204922
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DOI: https://doi.org/10.1007/BF00204922