Summary
For identification of semen in stain the specific activity of l-tartrate-inhibitable acid phosphatase (ACP) was determined. With each stain extract, both enzyme activity and protein concentration were determined, and the specific activity (enzyme activity/protein concentration) was calculated. Seminal stains showed a value of 23.8±15.2 (mean ± SD) IU/mg protein, while vaginal fluid stains showed a value of 0.088±0.049 IU/mg protein. Stains of other body fluids did not show any l-tartrate-inhibitable ACP activity. Furthermore, only eight of 30 plant juice stains showed any levels of l-tartrate-inhibitable ACP, although all plants tested showed ACP activity. As the present method enables us to analyze forensic samples quantitatively, it seems to be useful for forensic practice.
Zusammenfassung
Zur Identifizierung von Spermaspuren wurde die spezifische Aktivität der durch l-Tartrat hemmbaren sauren Phosphatase (ACP) bestimmt. Für jeden Spurenextrakt wurde die Proteinkonzentration sowie die spezifische Aktivität des Enzyms ermittelt. Spermaspuren wiesen Aktivitätswerte von 23,8±15,2 IU/mg Protein auf, Spuren von Vaginalflüssigkeit hingegen nur von 0,088±0,049 IU/mg Protein. Spuren anderer Körperflüssigkeiten zeigten diese Aktivität nicht. Darüber hinaus konnte nur bei 8 von 30 Spuren von Pflanzensäften Aktivitäten der durch l-Tartrat hemmbaren ACP nachgewiesen werden, obwohl alle untersuchten Pflanzen ACP-Aktivität besaßen. Die Methode scheint für die forensische Praxis geeignet, da sie quantitative Ergebnisse liefert.
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Tamaki, K., Fujisawa, K., Okajima, H. et al. Identification of semen in stain by determination of the specific activity of l-tartrate-inhibitable acid phosphatase. Z Rechtsmed 102, 429–436 (1989). https://doi.org/10.1007/BF00204073
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DOI: https://doi.org/10.1007/BF00204073
Key words
- Semen detection, acid phosphatase
- l-tartrate inhibitable acid phosphatase
- Identification of semen in stain