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Renal cysts and associated renal tumours in male ddY mice injected with ferric nitrilotriacetate

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Abstract

In experiments using ferric nitrilotriacetate (Fe-NTA) as a renal carcinogen, multiple renal cysts are often observed in addition to renal tumours. In the present study, we used 3-week-old male ddY mice and examined the relation between renal cysts and cancer development. Four months after the start of Fe-NTA administration, we observed cysts in the renal cortex in all Fe-NTA-treated mice, but not in Fe-free NTA-treated mice. Three types of cysts were observed, but only those which originated from the renal proximal tubules showed multi-layered or papillary growth of cyst epithelial cells. Using histochemical staining, we found a cyst formation-tumour induction sequence, and the supposed cystic-papillary tumour induced by Fe-NTA was of proximal tubular cell origin. We also found that the minimum dose of Fe-NTA capable of inducing renal tumours in ddY mice was 10 mg of iron/kg/day, four times in 2 weeks.

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Authors and Affiliations

  1. Department of Pathology, Okayama University Medical School, 2-5-1 Shikata-cho, 700, Okayama, Japan

    A. Kondo, J. Deguchi & S. Okada

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  1. A. Kondo
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  2. J. Deguchi
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  3. S. Okada
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Kondo, A., Deguchi, J. & Okada, S. Renal cysts and associated renal tumours in male ddY mice injected with ferric nitrilotriacetate. Vichows Archiv A Pathol Anat 427, 91–99 (1995). https://doi.org/10.1007/BF00203743

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  • DOI: https://doi.org/10.1007/BF00203743

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