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Immunocytochemical localization of allergenic protein (Ole e I) in the endoplasmic reticulum of the developing pollen grain of olive (Olea europaea L.)

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Abstract

The monoclonal antibody OL-1 and transmission electron microscopy were used to locate immunologically the major allergen of olive pollen. Ole e I, during pollen grain development. Within the pollen grain, allergenic proteins are located in the cisternae of the rough endoplasmic reticulum. Our findings indicate that the synthesis of these proteins starts in the vegetative cytoplasm of olive pollen during the early maturation stage.

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Abbreviations

MAb:

monoclonal antibody

rER:

rough endoplasmic reticulum

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Authors and Affiliations

  1. Departmento de Bioquímica, Biología Celular y Molecular de Plantas, Estación Experimental del Zaidín (CSIC), Profesor Albareda 1, E-18008, Granada, Spain

    M. I. Rodríguez-García, M. C. Fernández & J. D. Alché

Authors
  1. M. I. Rodríguez-García
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  2. M. C. Fernández
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  3. J. D. Alché
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Additional information

Dedicated to Professor Andreas Sievers on the occasion of his retirement

We wish to thank Ms. Matilde Garrido for technical help with the electron microscopy, Dr. C. Lahoz (Jimenez Diaz Foundation, Madrid, Spain) for kindly providing monoclonal antibody OL-1, and Ms. Karen Shashok for improving the English text of the manuscript. This work was supported by grant PB92-0079-CO3-03 from the Dirección General de Investigatión Cientifica y Técnica (DGICYT) and the Junta de Andalucía (Research Group # 2063), Spain.

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Rodríguez-García, M.I., Fernández, M.C. & Alché, J.D. Immunocytochemical localization of allergenic protein (Ole e I) in the endoplasmic reticulum of the developing pollen grain of olive (Olea europaea L.). Planta 196, 558–563 (1995). https://doi.org/10.1007/BF00203656

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  • DOI: https://doi.org/10.1007/BF00203656

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