Abstract
The activity of 1-acylglycerophosphocholine (1-acyl-GPC) O-acyltransferase (EC 2.3.1.23) varied during maturation of safflower (Carthamus tinctorius L.) seeds, and activity per seed was highest in the middle period of seed development when triacylglycerol (TAG) is most rapidly synthesized. The specific activity of acyl transfer in a 20000·g particulate preparation exceeded 500nmol·min-1·(mg protein)-1 and was higher than those of any other enzymes involved in TAG synthesis (K. Ichihara et al., 1993, Plant Cell Physiol. 34, 557–566). This suggested the presence of a large flux of acyl-CoA to phosphatidylcholine in the cell. The reaction was specific to C16 and C18 acyl-CoAs with a double bond at position 9. Lauroyl- and erucoyl-CoA were completely ineffective, while ricinoleoyl- and elaidoyl-CoA were utilized efficiently. The relative order of specificity for native acyl-CoA species was linoleoyl > oleoyl ≫ stearoyl = palmitoyl. When acyl-CoA mixtures were presented, preference for the unsaturated species rather than the saturated species was even more apparent. The enzyme preferentially utilized 1-C16-acyl- and 1-C18-acyl-GPC molecular species, and 1-palmitoyl-, 1-stearoyl-, 1-oleoyl-and 1-linoleoyl-GPC equally served as acyl acceptor. No activity was detected with 1-octanoyl-GPC, and 1-erucoyl-GPC produced little effect. The effectiveness of 1-alkyl-GPC was comparable to that of 1-acyl-GPC. It was thus concluded that the enzyme recognizes the chain lengths of the acyl donor and acceptor, and the double bond at position 9 of the acyl donor.
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Abbreviations
- DAG:
-
diacylglycerol
- DTNB:
-
5,5′-dithiobis(2-nitrobenzoic acid)
- GP:
-
sn-glycerol 3-phosphate
- GPC:
-
sn-glycero-3-phosphocholine
- GPE:
-
sn-glycero-3-phosphoethanolamine
- GPI:
-
sn-glycero-3-phosphoinositol
- PC:
-
phosphatidylcholine
- TAG:
-
triacylglycerol
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Ichihara, K., Mae, K., Sano, Y. et al. 1-Acylglycerophosphocholine O-acyltransferase in maturing safflower seeds. Planta 196, 551–557 (1995). https://doi.org/10.1007/BF00203655
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DOI: https://doi.org/10.1007/BF00203655