Abstract
The transport of [14C]glycyl-glycine (Gly-Gly) across the tonoplast of isolated barley vacuoles has been characterized. Uptake of the dipeptide Gly-Gly into barley mesophyll vacuoles was strongly increased by the addition of ATP, while Mg2+ inhibited the ATP-dependent fluxes. Inhibition of the vacuolar proton pump by bafilomycin or dissipation of the ΔpH had no effect on the ATP-dependent Gly-Gly uptake. Only the non-hydrolysable ATP analogue 5′-adenylylimidodiphosphate could partially substitute for ATP; ADP, GTP and UTP had no effect. Transport of Gly-Gly was saturable exhibiting an apparent K m of 49±5 mM. Uptake was inhibited by the sulphydryl reagents N-ethylmaleimide and p-chloromercuribenzenesulphonic acid. When present at 30 mM, various dipeptides as well as tri-glycine inhibited Gly-Gly (10 mM) uptake by 30–50%, whereas 5 mM phenylalanine was able to inhibit Gly-Gly uptake almost completely. Comparison with published data suggests that Gly-Gly is transferred across the tonoplast by the same system as several amino acids and inorganic ions.
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Abbreviations
- Gly-Gly:
-
glycyl-glycine
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We wish to thank Dr. J.-F. Chollet, Unité Associée CNRS 574, Université de Poitiers, Poitiers, France, for the synthesis of [14C]Gly-Gly and Professor N. Amrhein, Institut für Pflanzenwissenschaften, Federal Institute of Technology, Zürich, Switzerland, for critically reading the manuscript.
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Jamaï, A., Gaillard, C., Delrot, S. et al. Dipeptide transport in barley mesophyll vacuoles. Planta 196, 430–433 (1995). https://doi.org/10.1007/BF00203640
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DOI: https://doi.org/10.1007/BF00203640