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Use of two-dimensional protein-pattern analysis for the characterization of Arabidopsis thaliana mutants

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Abstract

Total proteins extracted from wild-type plants of Arabidopsis thaliana Heyhn, an etiolated mutant, a de-etiolated mutant and a mutant affected in cotyledon morphology, were analyzed by two-dimensional gel electrophoresis. Computer analysis of two-dimensional gels allowed the characterization of the mutants by a set of proteins showing a differential expression when compared with the wild-type plant grown under the same conditions. The overlap between comparisons of the diferent mutants with the wild-type allowed the identification of groups of polypeptides which, since their expression is altered in several mutants, might be involved in certain physiological functions. For example, this approach showed a possible involvement of actin in the elongation process. The simultaneous analysis of the two-dimensional protein patterns of different mutants seems, therefore, to be a promising approach to characterize proteins involved in various physiological functions.

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Abbreviations

2-D:

two-dimensional

IEF:

isoelectrofocusing

Mr :

relative molecular mass

E17.1:

E17 mutant grown in light

E22.1:

E22 mutant grown in light

E44.d:

E44 mutant grown in darkness

WT.d:

wild-type plants grown in darkness

WT.1:

Wild-type plants grown in light

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Authors and Affiliations

  1. I.N.R.A., Laboratoire de Biologie Cellulaire, route de Saint-Cyr, F-78026, Versailles Cedex, France

    Véronique Santoni, Catherine Bellini & Michel Caboche

Authors
  1. Véronique Santoni
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  2. Catherine Bellini
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  3. Michel Caboche
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Additional information

We would like to thank our laboratory colleagues J.A. Traas, H. Höfte and D. Bouchez (all from INRA) for useful discussions throughout this work. Also grateful thanks to Mr Zivy (Lab. Génétique des Sytèmes Végétaux. INRA. La Ferme du Moulon, Gif-sur-Yvette, France) for helpful discussions concerning 2-D gel analysis and to I. Small (Lab. Biologie Cellulaire. INRA, Versailles, France) for English text corrections.

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Santoni, V., Bellini, C. & Caboche, M. Use of two-dimensional protein-pattern analysis for the characterization of Arabidopsis thaliana mutants. Planta 192, 557–566 (1994). https://doi.org/10.1007/BF00203594

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  • DOI: https://doi.org/10.1007/BF00203594

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