Abstract
The auxin-binding protein designated ABP1 has been proposed to mediate auxin-induced cellular changes such as cell expansion. Its exact mode of action is unknown, but currently several approaches to elucidate its function are being pursued. One of these approaches, described here, is to determine the organ distribution of this putative auxin receptor in order to correlate spatially the abundance of the protein with some auxin-regulated activity such as cell elongation. The absolute and relative amounts of ABP1 were determined along the entire etiolated shoot, the root, and within the caryopsis of maize. ABP1 can be detected immunologically in all extracts of the etiolated maize seedling except the tip of the primary root and the endosperm. Within the shoot, but excluding the leaf roll, the highest levels compared on a fresh weight basis are in the apical mesocotyl and basal coleoptile regions, the areas of the most rapid cell elongation and the areas where there is the greatest capacity for auxin-induced growth. The relative abundance of ABP1 compared on a fresh weight basis changed more than fivefold in this organ. When compared on a total protein basis, the relative change in ABP1 abundance was approximately two-fold, which is less than the relative change in auxin-induced growth rate along the shoot. Differences in shoot growth rate among varieties of maize were compared with the relative amounts of ABP1 within the apical mesocotyl and basal coleoptile. A statistically significant but not perfect correlation was found between the auxin-induced growth rate of the apical mesocotyl and ABP1 abundance. These results demonstrate a general correlation between the amount of ABP1 and growth along the shoot and within maize hybrid varieties.
Abbreviations
- ABP1:
-
auxin-binding protein 1
- NAA:
-
naphthalene-1-acetic acid
- SDS:
-
sodium dodecyl sulfate
- PAGE:
-
poly-acrylamide gel electrophoresis.
References
Barbier-Brygoo H, Ephritikhine G, Klämbt D, Maurel C, Palme K, Schell J, Guern J (1991) Perception of the auxin signal at the plasma membrane of tobacco mesophyll protoplasts. Plant J 1:83–93
Bradford MM (1976) A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254
Cleland R (1964) The role of endogenous auxin in the elongation of Avena leaf sections. Physiol Plant 17:126–135
Davies PJ (1987) Plant hormones and their role in plant growth and development. Martinus Nijhoff, Boston
Fukuda H (1994) Redifferentiation of single mesophyll cells into tracheary elements. Int J Plant Sci 155:262–271
Goldsmith MH (1993) Cellular signaling: New insights into the action of the plant growth hormone auxin. Proc Natl Acad Sci USA 90:11442–11445
Hesse T, Felds wisch J, Balshusemann D, Bauw G, Puype G, Vanderkerckhove J, Löbler M, Klämbt D, Schell J, Palme K (1989) Molecular cloning and structural analysis of a gene from Zea mays (L.) coding for a putative receptor for the plant hormone auxin. EMBO J 8:2453–2461
Inohara N, Shimomura S, Fukui T, Futai M (1989) Auxin-binding protein located in the endoplasmic reticulum of maize shoots: Molecular cloning and complete primary structure. Proc Natl Acad Sci USA 86:3564–3568
Jones AM (1994) Auxin-binding proteins. Annu Rev Plant Physiol Plant Mol Biol 45:393–420
Jones AM, Cochran DS, Lamerson PL, Cohen J, Evans M (1991) Red-light-induced changes in auxin, an auxin-binding protein, and auxin transport in maize mesocotyl. Plant Physiol 97:352–358
Jones AM, Lamerson P, Venis MA (1989) Comparison of site I auxin binding and a 22-kilodalton auxin-binding protein in maize. Planta 179:409–414
Konigsberg WH, Henderson L (1983) Removal of sodium dodecyl sulfate from proteins by ion-pair extraction. Methods Enzymol 91:254–259
Löbler M, Klämbt D (1985) Auxin-binding proteins of corn (Zea mays L). I. Purification by immunological methods and characterization. J Biol Chem 260:9848–9853
Radermacher E, Klämbt D (1993) Auxin-dependent growth and auxin-binding proteins in primary root and root hairs of corn (Zea Mays L.). J Plant Physiol 141:689–703
Ray PM, Dohrmann U, Hertel R (1977) Specificity of auxin-binding sites on maize coleoptile membranes as possible receptor sites for auxin action. Plant Physiol 60:585–591
Tillmann U, Viola G, Kayser B, Siemester G, Hesse T, Palme K, Löbler M, Klämbt D (1989) cDNA clones of the auxin-binding protein from corn coleoptiles (Zea Mays L.): Isolation and characterization by immunological methods. EMBO J 8:2463–2467
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Harnden, D., Jones, A.M. Organ distribution of auxin-binding protein 1 in the etiolated maize seedling. J Plant Growth Regul 14, 109–113 (1995). https://doi.org/10.1007/BF00203122
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DOI: https://doi.org/10.1007/BF00203122