Abstract
Precise time and temperature dependences of the decrease of metabolism of cultured cells of tomato (Lysopersicon esculentum (L.) Mill. L. peruvianum (L.) Mill.) resulting from exposures to high and low temperatures were determined. Equations of the form Ln (activity)= C +1 [A+(T-Tm)N+B] describe thermal inactivation and allow prediction of activity loss following any thermal excursion beyond limits of temperature stability. The experimental parameters A, B, C and N derived from these equations allow precise comparison of temperature sensitivities of cells. Analysis of metabolic heat rates, O2-consumption rates and CO2-evolution rates demonstrated simultaneous shifts in metabolic pathways and metabolic activities towards more anaerobic metabolism below about 12° C and at high temperatures that stress growth of tomato cells.
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Breidenbach, R.W., Rank, D.R., Fontana, A.J., Hansen, L.D., Criddle, R.S. (1990) Calorimetric determination of tissue responses to thermal extremes as a function of time and temperature. Thermochim. Acta 172, 179–189
Criddle, R.S., Hansen, L.D. (1990) Metabolic rate of barley roots as a continuous function of temperature. J. Plant Physiol., in press
Criddle, R.S., Breidenbach, R.W., Lewis, E.A., Eatough, D.J., Hansen, L.D. (1988) Effects of temperature and oxygen depletion on metabolic rates of tomato and carrot cell cultures and cuttings measured by calorimetry. Plant Cell Environ. 11, 695–701
Criddle, R.S., Breidenbach, R.W., Rank, D.R., Hopkin, M.S., Hansen, L.D. (1990) Simultaneous calorimetric and respirometric measurements on plant tissues. Thermochim. Acta 172, 213–221
Criddle, R.S., Fontana, A.J., Rank, D.R., Paige, D., Hansen, L.D., Breidenbach, R.W. (1991) Simultaneous measurement of metabolic heat rate, CO2 production, and O2 consumption by microcalorimetry. Anal. Biochem. 194, 413–417
Fontana, A.J., Hansen, L.D., Breidenbach, R.W., Criddle, R.S. (1990) Microcalorimetric measurement of aerobic cell metabolism in unstirred cell cultures. Thermochim. Acta 172, 105–113
Graham, D., Patterson, B.D. (1982) Responses of plants to low, nonfreezing temperatures: proteins, metabolism, and acclimation. Annu. Rev. Plant Physiol. 33, 347–372
Hansen, L.D., Criddle, R.S. (1990) Determination of phase changes and metabolic rates in plant tissues as a function of temperature by heat conduction DSC. Thermochim. Acta 160, 173–192
Levitt, J. (1980) Responses of plants to environmental stresses, vol. 1. Academic Press, New York
Lyons, J.M., Breidenbach, R.W. (1989) Relation of chilling stress to respiration. In: Chilling injury of horticultural crops, pp. 223–233, Yang, C.Y., ed. CRC Press, Boca Raton, Fla., USA
Raison, J.K. (1980) Effect of low temperature on respiration. In: The biochemistry of plants, vol. 2, chpt. 15, Davies, D.D., ed. Academic Press, New York
Thomas, B.R., Pratt, D. (1981) efficient hybridization between Lycopersicon esculentum and L. peruvianum via embryo callus. Theor. Appl. Genet. 69, 215–219
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We gratefully acknowledge support for this project by the U.S. Department of Agriculture Competitive Research Grants Office, grant 90-37280-5592. Allied support by Pioneer HiBred Inc., the California Rice Research Board, and Dole Package Foods Co., Division of Castle and Cooke Co., has also been helpful to this work.
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Rank, D.R., Breidenbach, R.W., Fontana, A.J. et al. Time-temperature responses of tomato cells during high- and low-temperature inactivation. Planta 185, 576–582 (1991). https://doi.org/10.1007/BF00202969
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DOI: https://doi.org/10.1007/BF00202969