Skip to main content
SpringerLink
Log in

Expression of epidermal growth factor and its receptor in rabbits with ischaemic acute renal failure

  • Original Article
  • Published:
Virchows Archiv Aims and scope Submit manuscript

Abstract

Urinary immunoreactive epidermal growth factor (EGF) levels decrease, and renal immunoreactive EGF levels increase in rats with ischaemic acute renal failure (ARF). We investigated the immunohistochemical localization of EGF and EGF receptor in rabbits with ischaemic ARF to clarify the significance of renal EGF. Male New Zealand White rabbits underwent right nephrectomy prior to a 60 min renal artery clamp. At 3, 6, 24, 48, 72 and 96 h after ischaemia, serum urea nitrogen and serum creatinine were determined. Guinea pig anti-rabbit EGF antibody and monoclonal anti-EGF receptor antibody were used for the primary incubation. EGF was immunolocalized to the ascending limb of Henle and the distal convoluted tubule in the normal right kidneys. However, in the post ischaemic left kidneys at 6, 24, 48 and 72 h, immunoreactivity of EGF was associated with proximal tubules. In the normal kidneys, antibody to EGF receptor reacted with distal tubules and collecting ducts. In the ischaemic kidneys, EGF receptor was localized in the basolateral membrane in the proximal tubules. The expression of EGF and EGF receptor in renal tubules may play an important role in repair following ischaemic renal damage.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Behrens MT, Corbin AL, Hise MK (1989) Epidermal growth factor receptor regulation in rat kidney: two models of renal growth. Am J Physiol 257:F1059-F1064

    Google Scholar 

  2. Boitnott JK, Solez K (1984) The use of ranking in the statistical assessment of morphologic data. Acute renal failure: correlations between morphology and function. In: Solez K, Whelton A (eds) Dekker, New York, pp 13–16

    Google Scholar 

  3. Breyer MD, Jacobson HR, Breyer JA (1988) Epidermal growth factor inhibits the hydroosmotic effect of vasopressin in the isolated perfused rabbit cortical collecting tubule. J Clin Invest 82:1313–1320

    Google Scholar 

  4. Carpenter G, Cohen S (1979) Epidermal growth factor. Annu Rev Biochem 48:193–216

    Article  CAS  PubMed  Google Scholar 

  5. Cohen S (1962) Isolation of a mouse submaxillary gland protein accelerating incisor eruption and eyelid opening in the new-born animal. J Biol Chem 237:1555–1562

    Google Scholar 

  6. Cohen S (1987) Epidermal growth factor. In vitro cell. Dev Biol 23:239–246

    Google Scholar 

  7. Cuppage FE, Chiga M, Tate A (1972) Cell cycle studies in the regenerating rat nephron following injury with mercuric chloride. Lab Invest 26:122–126

    Google Scholar 

  8. Dailey GE, Kraus JW, Orth DN (1978) Homologous radioimmunoassay for human epidermal growth factor (urogastrone). J Clin Endocrinol Metab 46:929–936

    Google Scholar 

  9. Fisher DA, Salido EC, Barajas L (1989) Epidermal growth factor and the kidney. Annu Rev Physiol 51:67–80

    Google Scholar 

  10. Hallett CJ, Cook JGH (1971) Reduced nicotinamide adenine dinucleotide-coupled reaction for emergency blood urea estimation. Clin Chim Acta 35:33–37

    Google Scholar 

  11. Harris RC, Hoover RL, Jacobson HR, Badr KF (1988) Evidence for glomerular actions of epidermal growth factor in the rat. J Clin Invest 82:1028–1039

    Google Scholar 

  12. Hirata Y, Orth DN (1979) Epidermal growth factor (urogastrone) in human tissues. J Clin Endocrinol Metab 48:667–672

    Google Scholar 

  13. Humes HD, Cieslinski DA, Coimbra TM, Messana JM, Galvao C (1989) Epidermal growth factor enhances renal tubule cell regeneration and repair and accelerates the recovery of renal function in postischemic acute renal failure. J Clin Invest 84:1757–1761

    Google Scholar 

  14. Humes HD, Beals TF, Cieslinski DA, Sanchez IO, Page TP (1991) Effects of transforming growth factor-β, transforming growth factor-α, and other growth factors on renal proximal tubule cells. Lab Invest 64:538–545

    Google Scholar 

  15. Humes HD, Cieslinski DA, Johnson LB, Sanchez IO (1992) Triiodothyronine enhances renal tubule cell replication by stimulating EGF receptor gene expression. Am J Physiol 262:F540-F545

    Google Scholar 

  16. Kajikawa K, Yasui W, Sumiyoshi H, Yoshida K, Nakayama H, Ayhan A, Yokozaki H, Ito H, Tahara E (1991) Expression of epidermal growth factor in human tissues: Immunohistochemical and biochemical analysis. Virchows Arch [A] 418:27–32

    Google Scholar 

  17. Kawai S, Matsubara A, Oshiden K, Matsuo Y, Yasuda S, Hong Y-M, Yanaihara N (1989) Isolation and sequence analysis of EGF from rabbit urine. In: Ueki M (ed) Peptide chemistry. Protein Research Foundation, Osaka, pp 169–172

    Google Scholar 

  18. Mathiesen ER, Nexø E, Hommel E, Parving H-H (1989) Reduced urinary excretion of epidermal growth factor in incipient and overt diabetic nephropathy. Diabetic Med 6:121–126

    Google Scholar 

  19. Mattila A-L, Pasternack A, Viinikka L, Perheentupa J (1986) Subnormal concentrations of urinary epidermal growth factor in patients with kidney disease. J Clin Endocrinol Metab 62:1180–1183

    Google Scholar 

  20. Norman J, Badie-Dezfooly B, Nord EP, Kurtz I, Schlosser J, Chaudhari A, Fine LG (1987) EGF-induced mitogenesis in proximal tubular cells: potentiation by angiotensin II. Am J Physiol 253:F299-F309

    Google Scholar 

  21. Norman J, Tsau Y-K, Bacay A, Fine LG (1990) Epidermal growth factor accelerates functional recovery from ischaemic acute tubular necrosis in the rat: role of the epidermal growth factor receptor. Clin Sci 78:445–450

    Google Scholar 

  22. Ohgai H, Kumakura T, Komoto S, Matsuo Y, Oshiden K, Koide T, Yanaihara C, Yanaihara N (1989) Production of rat epidermal growth factor by Escherichia coli cells containing a secretion plasmid. J Biotechnol 10:151–160

    Google Scholar 

  23. Rall LB, Scott J, Bell GI, Crawford RJ, Penschow JD, Niall HD, Coghlan JP (1985) Mouse prepro-epidermal growth factor synthesis by the kidney and other tissues. Nature 313:228–231

    Google Scholar 

  24. Safirstein R, Zelent AZ, Price PM (1989) Reduced renal prepro-epidermal growth factor mRNA and decreased EGF excreation in ARF. Kidney Int 36:810–815

    Google Scholar 

  25. Safirstein R, Price PM, Saggi SJ, Harris RC (1990) Changes in gene expression after temporary renal ischemia. Kidney Int 37:1515–1521

    Google Scholar 

  26. Salido EC, Yen PH, Shapiro LJ, Fisher DA, Barajas L (1989) In situ hybridization of prepro-epidermal growth factor mRNA in the mouse kidney. Am J Physiol 256:F632-F638

    Google Scholar 

  27. Schaudies RP, Johnson JP (1993) Increased soluble EGF after ischemia is accompanied by a decrease in membrane-associated precursors. Am J Physiol 264:F523-F531

    Google Scholar 

  28. Schaudies RP, Nonclercq D, Nelson L, Toubeau G, Zanen J, Heuson-Stiennon J-A, Laurent G (1993) Endogenous EGF as a potential renotrophic factor in ischemia-induced acute renal failure. Am J Physiol 265:F425-F434

    Google Scholar 

  29. Solez K, Morel-Maroger L, Sraer J-D (1979) Morphology of “acute tubular necrosis” in man: analysis of 57 renal biopsies and comparison with the glycerol model. Medicine 58:362–376

    Google Scholar 

  30. Stanton RC, Seifter JL (1988) Epidermal growth factor rapidly activates the hexose monophosphate shunt in kidney cells. Am J Physiol 253:C267-C271

    Google Scholar 

  31. Starkey RH, Orth DN (1977) Radioimmunoassay of human epidermal growth factor (urogastrone). J Clin Endocrinol Metab 45:1144–1153

    Google Scholar 

  32. Taira T, Yoshimura A, Iizuka K, Iwasaki S, Ideura T, Koshikawa S (1993) Urinary epidermal growth factor levels in patients with acute renal failure. Am J Kidney Dis 22:656–661

    Google Scholar 

  33. Taira T, Yoshimura A, Inui K, Oshiden K, Ideura T, Koshikawa S, Solez K (1994) Immunochemical study of epidermal growth factor in rats with mercuric chloride-induced acute renal failure. Nephron 67:88–93

    Google Scholar 

  34. Toffaletti J, Blosser N, Hall T, Smith S, Tompkins D (1983) An automated dry-slide enzymatic method evaluated for measurement of creatinine in serum. Clin Chem 29:684–687

    Google Scholar 

  35. Toubeau G, Nonclercq D, Zanen J, Lambricht P, Tulkens PM, Heuson-Stiennon JA, Laurent G (1991) Distribution of epidermal growth factor in the kidneys of rats exposed to amikacin. Kidney Int 40:691–699

    Google Scholar 

  36. Yanaihara N, Yanaihara C, Nokihara K, Iguchi K, Fukata S, Tanaka M, Yamamoto Y, Mochizuki T (1984) Immunochemical study on PHI/PHM with use of synthetic peptides. Peptides 5:247–254

    Google Scholar 

  37. Yoshioka K, Takemura T, Murakami K, Akano N, Matsubara K, Aya N, Maki S (1990) Identification and localization of epidermal growth factor and its receptor in the human glomerulus. Lab Invest 63:189–196

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Internal Medicine, Showa University Fujigaoka Hospital, 1-30 Fujigaoka, Aoba-ku, 227, Yokohama, Japan

    T. Taira, A. Yoshimura, K. Iizuka, K. Inui, S. Iwasaki, T. Ideura & S. Koshikawa

  2. Ako Laboratory, Otsuka Pharmaceutical Co., Ako, Japan

    K. Oshiden

Authors
  1. T. Taira
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. Yoshimura
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. K. Iizuka
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. K. Inui
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. S. Iwasaki
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. T. Ideura
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. S. Koshikawa
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. K. Oshiden
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Taira, T., Yoshimura, A., Iizuka, K. et al. Expression of epidermal growth factor and its receptor in rabbits with ischaemic acute renal failure. Vichows Archiv A Pathol Anat 427, 583–588 (1996). https://doi.org/10.1007/BF00202889

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00202889

Key words

Search

Navigation