Abstract
The origin of the quiescent center in the embryonic radicle of Capsella bursa-pastoris was investigated by in-situ hybridization to cellular polyadenylic-acid-containing RNA using [3H]polyuridylic acid as a probe. In the globular embryo, autoradiographic silver grains were localized in all cells of the presumptive root apex except in the hypophysis. As the inner cell formed by a transverse division of the hypophysis cut off new cells toward the central procambial cylinder of the embryo, these cells remained characteristically unlabeled, in contrast to the labeled cells of the rest of the embryo. In the embryonic radicles of mature seeds and of seedlings, cells derived from the hypophysis appeared as a nonmeristematic, unlabeled, hemispherical group, bounded by the procambium to the inside and the root epidermis to the outside. When root tips excised from 2-d-old seedlings were incubated in [methyl-3H]thymidine, sectioned, and autoradiographed, cells derived from the inner cell of the hypophysis were found to be unlabeled, thus showing that they constitute the specific cells of the quiescent center. These results present evidence for the single-cell origin of the quiescent center in an angiosperm root and a role for the hypophysis in it.
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Abbreviations
- poly(A)+RNA:
-
polyadenylicacid-containing RNA
- [3H]poly(U):
-
[3H]polyuridylic acid
- QC:
-
quiescent center
References
Alfieri, I.R., Evert, R.F. (1968) Analysis of meristematic activity in the root tip of Melilotus alba Desr. New Phytol. 67, 641–647
Beers, R.F. (1960) Hydrolysis of polyadenylic acid by pancreatic ribonuclease. J. Biol. Chem. 235, 2393–2398
Capco, D.G., Jeffery, W.R. (1978) Differential distribution of poly(A)-containing RNA in the embryonic cells of Oncopeltus fasciatus. Analysis by in situ hybridization. Dev. Biol. 67, 137–151
Clowes, F.A.L. (1956) Nucleic acids in root apical meristems of Zea. New Phytol. 55, 29–34
Clowes, F.A.L. (1958) Protein synthesis in root meristems. J. Exp. Bot. 9, 229–238
Clowes, F.A.L. (1961) Apical meristems. Blackwell, Oxford, UK
Clowes, F.A.L. (1975) The quiescent center. In: The development and function of roots, pp. 3–19, Torrey, J.G., Clarkson, D.T., eds. Academic Press, London
Clowes, F.A.L. (1978a) Origin of the quiescent centre in Zea mays. New Phytol. 80, 409–419
Clowes, F.A.L. (1978b) Origin of quiescence at the root pole of pea embryos. Ann. Bot. 42, 1237–1239
Clowes, F.A.L., Juniper, B.E. (1964) The fine structure of the quiescent centre and neighboring tissues in root meristems. J. Exp. Bot. 15, 622–630
Darnell, J.E., Wall, R., Tushinski, R.J. (1971) An adenylic acid-rich sequence in messenger RNA of HeLa cells and its possible relationship to reiterated sites in DNA. Proc. Natl. Acad. Sci. USA 68, 1321–1325
Deschamps, R. (1969) Premiers stades du développement de l'embryon et de l'albumen du lin: étude au microscope electronique. Rev. Cytol. Biol. Vég. 32, 379–390
Feder, N., O'Brien, T.P. (1968) Plant microtechnique: some principles and new methods. Am. J. Bot. 55, 123–142
Feldman, L.J. (1976) The de-novo origin of the quiescent center in regenerating root apices of Zea mays. Planta 128, 207–212
Fisher, D.B. (1968) Localization of endogenous RNA polymerase activity in frozen sections of plant tissues. J. Cell Biol. 39, 745–749
Gifford, E.M., Foster, A.S. (1989) Morphology and evolution of vascular plants, 3rd edn. W.H. Freeman, New York
Jensen, W.A. (1958) The nucleic acid and protein content of root tip cells of Vicia faba and Allium cepa. Exp. Cell Res. 14, 575–583
Jones, P.A. (1977) Development of the quiescent center in maturing embryonic radicles of pea (Pisum sativum L. cv. Alaska). Planta 135, 233–240
Juniper, B.E., Barlow, P.W. (1969) The distribution of plasmodesmata in the root tip of maize. Planta 89, 352–360
Maheshwari, P. (1950) An introduction to the embryology of angiosperms. McGraw-Hill, New York
Phillips, H.L., Jr., Torrey, J.G. (1974) The ultrastructure of the quiescent center in the apex of cultured roots of Convolvulus arvensis L. Am. J. Bot. 61, 871–878
Porter, E.K., Parry, D., Dickinson, H.G. (1983) Changes in poly(A)+RNA during male meiosis in Lilium. J. Cell Sci. 62, 177–186
Raghavan, V. (1981a) A transient accumulation of poly(A)-containing RNA in the tapetum of Hyoscyamus niger during microsporogenesis. Dev. Biol. 81, 342–348
Raghavan, V. (1981b) Distribution of poly(A)-containing RNA during normal pollen development and during induced pollen embryogenesis in Hyoscyamus niger. J. Cell Biol. 89, 593–606
Raghavan, V. (1987) Changes in poly(A)+RNA concentrations during germination of spores of the fern, Onoclea sensibilis. Protoplasma 140, 55–66
Raghavan, V., Okonkwo, S.N.C. (1982) Studies on the germination of seeds of the root parasites, Alectra vogelii and Striga gesnerioides. II. DNA synthesis and development of the quiescent center in the radicle. Am. J. Bot. 69, 1646–1656
Raghavan, V., Olmedilla, A. (1989) Spatial patterns of histone mRNA expression during grain development and germination in rice. Cell Differ. Dev. 27, 183–196
Raghavan, V., Torrey, J.G. (1963) Growth and morphogenesis of globular and older embryos of Capsella in culture. Am. J. Bot. 50, 540–551
Rondet, P. (1962) L'organogenèse au cours de l'embryogenèse chez l'Alyssum maritimum Lamk. C.R. Acad. Sci. Paris 255, 2278–2280
Schaffner, M. (1906) The embryology of the Shepherd's purse. Ohio Natural. 7, 1–8
Schulz, R., Jensen, W.A. (1968) Capsella embryogenesis: the early embryo. J Ultrastruct. Res. 22, 376–392
Simoncioli, C. (1974) Ultrastructural characteristics of “Diplotaxis erucoides (L.) DC.” suspensor. G. Bot. Ital. 108, 175–189
Snedecor, G.W. (1956) Statistical methods, 5th edn. The Iowa State College Press, Ames, USA
Souèges, R. (1919) Les premières divisions de l'oeuf et les différenciation du suspenseur chex le Capsella bursa-pastoris Moench (1). Ann. Sci. Nat. Bot. Ser. X 1, 1–28
Sterling, C. (1955) Embryogeny in the lima bean. Bull. Torrey Bot. Club 82, 325–338
Vallade, J. (1972) Structure et fonctionnement du méristème lors de la formation de la jeune racine primaire chez un Petunia hybrida hort. C.R. Acad. Sci. Paris 274D, 1027–1030
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This work was supported in part by National Science Foundation grants PCM-7902898 and DCB-8709092.
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Raghavan, V. Origin of the quiescent center in the root of Capsella bursa-pastoris (L.) Medik. Planta 181, 62–70 (1990). https://doi.org/10.1007/BF00202325
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DOI: https://doi.org/10.1007/BF00202325