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Lipoxygenase heterogeneity in Pisum sativum

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Abstract

Antibodies raised against two pea (Pisum sativum L. cv. Birte) seed lipoxygenases have been used to analyze lipoxygenase heterogeneity in seeds and in other organs. At least seven different polypeptides were identified in vivo; five of these were identified as precursors synthesized in vitro. The developmental appearance of the seed polypeptides has been analyzed and ‘early’ and ‘late’ forms were identified. Limited N-terminal sequence data indicated further heterogeneity when compared with sequences predicted from cDNAs.

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Abbreviations

cDNA:

complementary DNA

DAF:

days after flowering

HPLC:

high-performance liquid chromatography

Ig:

immunoglobulin

kb:

kilobase

Mr :

relative molecular mass

PAGE:

polyacrylamide gel electrophoresis

PVDF:

polyvinylidene difluoride

SDS:

sodium dodecyl sulphate

SSC:

0.15 M sodium chloride, 0.015 M sodium citrate, pH 7.0

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Authors and Affiliations

  1. John Innes Institute, Colney Lane, NR4 7UH, Norwich, UK

    C. Domoney, J. L. Firmin, P. M. Ealing & R. Casey

  2. Unilever Research, Colworth Laboratory, MK44 1LQ, Sharnbrook, Bedford, UK

    C. Sidebottom & A. Slabas

Authors
  1. C. Domoney
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  2. J. L. Firmin
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  3. C. Sidebottom
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  4. P. M. Ealing
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  5. A. Slabas
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  6. R. Casey
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Additional information

This work was supported by the Agricultural and Food Research Council via a grant-in-aid to the John Innes Institute. We acknowledge financial support from the Commission of the European Communities Biotechnology Action Programme; grant No. 0063-UK.

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Domoney, C., Firmin, J.L., Sidebottom, C. et al. Lipoxygenase heterogeneity in Pisum sativum . Planta 181, 35–43 (1990). https://doi.org/10.1007/BF00202322

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  • DOI: https://doi.org/10.1007/BF00202322

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