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Phytotropin-binding sites and auxin transport in Cucurbita pepo: evidence for two recognition sites

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Abstract

Two properties of phytotropins, their ability to bind to 1-N-naphthylphthalamic acid (NPA) receptors located on microsomal vesicles isolated from Cucurbita pepo L. hypocotyls, and to stimulate auxin (indol-3-yl acetic acid, IAA) accumulation into such vesicles by blocking its efflux from them, were assessed in double labelling experiments using [2,3,4,5-3H]1-N-naphthylphthalamic acid and 3-indolyl-[2-14C]acetic acid. Two sites of differing affinities and activities on IAA accumulation were found. 1-N-Naphthylphthalamic acid was found to have high affinity (KD at 10-8mol·l-1) for one site and low affinity (KD at 10-6 mol·l-1) for the other, whereas 2-(1-pyrenoyl)benzoic acid displaced NPA with high efficiency (KD below 10-8 mol·l-1) from both sites. Other phytotropins had intermediate affinities for either site. Occupation of the site with low affinity for NPA stimulated auxin accumulation, while occupation of the high-affinity site with a phytotropin did not interfere with auxin accumulation into vesicles.

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Abbreviations

IAA:

Indol-3-yl acetic acid

NPA:

1-N-naphthylphthalamic acid

PBA:

2-(1-pyrenoyl)benzoic acid

TIBA:

2,3,5-triiodobenzoic acid

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Authors and Affiliations

  1. Institut für Biologie III der Universität, W-7800, Freiburg, Federal Republic of Germany

    Wolfgang Michalke

  2. CSIRO Division of Plant Industry, P.O. Box 1600, 2601, Canberra City, A.C.T., Australia

    Gerard F. Katekar & Art E. Geissler

Authors
  1. Wolfgang Michalke
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  2. Gerard F. Katekar
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  3. Art E. Geissler
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Additional information

W.M. was supported in part by an allowance from CSIRO and in part by a fellowship of the Deutsche Forschungsgemeinschaft; he acknowledges the friendly hospitality of the CSIRO Division of Plant Industry. The authors thank R. Hertel (Freiburg) for valuable discussion.

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Michalke, W., Katekar, G.F. & Geissler, A.E. Phytotropin-binding sites and auxin transport in Cucurbita pepo: evidence for two recognition sites. Planta 187, 254–260 (1992). https://doi.org/10.1007/BF00201948

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