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Linkage mapping of the tumor necrosis factor receptor 2 (TNFR2) gene to 1p36.2 using the single-strand conformation polymorphism technique

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Abstract

We identified two informative polymorphisms in the transcribed 3′ untranslated region of the tumor necrosis factor receptor 2 (TNFR2) gene using the poly-merase chain reaction (PCR) with the single-strand conformation polymorphism (SSCP) technique. These poly-morphisms demonstrated Mendelian inheritance and were useful for linkage analysis. TNFR2 was very closely linked to the pronatriodilatin gene (PND), and the TNFR2 SSCP polymorphisms were much more informative than the restriction fragment length polymorphisms currently available for the PND locus. In addition, we have demonstrated that genotyping could be performed with DNA obtained from paraffin-embedded tissue.

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Author notes

  1. Peter S. White

    Present address: Division of Oncology, Children's Hospital of Philadelphia, 34th Civic Ctr. Blvd., 19104, Philadelphia, Pennsylvania, USA

Authors and Affiliations

  1. Department of Neurological Surgery, Washington University School of Medicine, Saint Louis Children's Hospital, 1 Children's Place, 63110, Saint Louis, MO, USA

    Bruce A. Kaufman

  2. Department of Pediatrics, Washington University School of Medicine, Saint Louis Children's Hospital, 1 Children's Place, 63110, Saint Louis, MO, USA

    Peter S. White & Garrett M. Brodeur

  3. Department of Surgery, Washington University School of Medicine, Saint Louis, Missouri, USA

    Todd Steinbrueck & Helen Donis-Keller

Authors
  1. Bruce A. Kaufman
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  2. Peter S. White
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  3. Todd Steinbrueck
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  4. Helen Donis-Keller
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  5. Garrett M. Brodeur
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Kaufman, B.A., White, P.S., Steinbrueck, T. et al. Linkage mapping of the tumor necrosis factor receptor 2 (TNFR2) gene to 1p36.2 using the single-strand conformation polymorphism technique. Hum Genet 94, 418–422 (1994). https://doi.org/10.1007/BF00201604

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  • DOI: https://doi.org/10.1007/BF00201604

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