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A method for transforming lymphocytes from very small blood volumes suitable for paediatric samples

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Summary

Permanent lymphoblastoid cell lines are important in the molecular analysis and characterization of human genetic disorders, when immortalized cells must be banked for future diagnostic or research purposes. However, routine methods for transformation using Epstein-Barr virus (EBV) require blood volumes that may be difficult to collect from clinically compromised neonates and small children. Here we report a modified transformation procedure utilizing blood samples of small volume (less than 1.0ml), which we have found to be particularly useful for the immortalization of lymphocytes destined for future molecular genetic studies.

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References

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Authors and Affiliations

  1. Molecular Biology Program, Child Health Research Institute and Division of Medical Genetics, Department of Paediatrics, Canada

    J. Elliott, M. B. Coulter-Mackie, J. H. Jung, D. I. Rodenhiser & S. M. Singh

  2. Children's Psychiatric Research Institute, Canada

    M. B. Coulter-Mackie

  3. Department of Zoology, University of Western Ontario, N6C 2V5, London, Ontario, Canada

    S. M. Singh

Authors
  1. J. Elliott
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  2. M. B. Coulter-Mackie
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  3. J. H. Jung
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  4. D. I. Rodenhiser
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  5. S. M. Singh
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Elliott, J., Coulter-Mackie, M.B., Jung, J.H. et al. A method for transforming lymphocytes from very small blood volumes suitable for paediatric samples. Hum Genet 86, 615–616 (1991). https://doi.org/10.1007/BF00201551

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  • DOI: https://doi.org/10.1007/BF00201551

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