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Stretch-activated chloride, potassium, and calcium channels coexisting in plasma membranes of guard cells of Vicia faba L.

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Abstract

Mechanosensitive ion channels in the plasma membrane of Vicia faba guard cell protoplasts were studied by use of the patch clamp technique. Stretch-activated (SA) channels in outside-out patches were analyzed for channel conductance, kinetics and ion selectivity. We found three distinct SA channels, permeable to Cl−, K+ and Ca2+ and distinguishable from spontaneous (non-SA) channels for these ions on the basis of conductance, kinetics, and voltage-dependence, as well as sensitivity to membrane stretch. In the attached patch configuration, light suction (2 to 10 kPa) reversibly induced channel opening with multiple amplitudes and complex kinetics. The open probability for SA channels increased nonlinearly with pipette suction. In guard cells in situ, these SA channels may mediate ion transport across the plasma membrane directly, as well as influence the activity of non-SA channels via effects on membrane voltage and cytoplasmic calcium. Through such effects, SA channels likely influence volume and turgor regulation of guard cells, and thereby control of leaf gas exchange.

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Abbreviations

EK:

equilibrium potential for potassium transport

ECl :

equilibrium potential for chloride transport

SA:

stretchactivated

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Author notes

  1. Daniel J. Cosgrove

    Present address: Department of Biology, 208 Mueller Laboratory, Pennsylvania State University, 16802, University Park, PA, USA

  2. Rainer Hedrich

    Present address: Institut für Biophysik, Universität Hannover, Herrenhäuser Strasse 2, W-3000, Hannover 21, Germany

Authors and Affiliations

  1. Pflanzenphysiologisches Institut der Universität, Untere Karspüle 2, W-3400, Göttingen, Germany

    Daniel J. Cosgrove & Rainer Hedrich

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  1. Daniel J. Cosgrove
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  2. Rainer Hedrich
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Additional information

Dedicated to the 80. birthday of Franz Hedrich

Supported by a grant from the Deutsche Forschungsgemeinschaft to R.H. and a Department of Energy grant to D.J.C. gratefully acknowledges a John S. Guggenheim Fellowship and Fulbright Kommission Senior Professor Award. We thank Ingrid Baumann and Angela Schön for technical assistance, and Klaus Raschke and Heiner Busch for spirited discussions and support.

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Cosgrove, D.J., Hedrich, R. Stretch-activated chloride, potassium, and calcium channels coexisting in plasma membranes of guard cells of Vicia faba L.. Planta 186, 143–153 (1991). https://doi.org/10.1007/BF00201510

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  • DOI: https://doi.org/10.1007/BF00201510

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