Abstract
Detached leaves of Bryophyllum fedtschenkoi Hamet et Perrier kept in normal air show a single period of net CO2 fixation on transfer to constant darkness at temperatures in the range 0–25 °C. The duration of this initial fixation period is largely independent of temperature in the range 5–20 °C, but lengthens very markedly at temperatures below 4 °C, and is reduced at temperatures above 25 °C. The onset of net fixation of CO2 on transfer of leaves to constant darkness is immediate at low temperatures, but is delayed as the temperature is increased. The ambient temperature also determines whether or not a circadian rhythm of CO2 exchange occurs. The rhythm begins to appear at about 20 °C, is most evident at 30 °C and becomes less distinct at 35 °C. The occurrence of a distinct circadian rhythm in CO2 output at 30° C in the absence of a detectable rhythm in PEPCase kinase activity shows that the kinase rhythm is not a mandatory requirement for the rhythm of PEPCase activity. However, when it occurs, the kinase rhythm undoubtedly amplifies the PEPCase rhythm.
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Abbreviations
- PEPCase:
-
phosphoenolpyruvate carboxylase
References
Anderson, C.M., Wilkins, M.B. (1989a) Period and phase control by temperature in the circadian rhythm of CO2 exchange in illuminated leaves of Bryophyllum fedtschenkoi. Planta 177, 456–469
Anderson, C.M., Wilkins, M.B. (1989b) Phase resetting of the circadian rhythm of carbon-dioxide assimilation in Bryophyllum leaves in relation to their malate content following brief exposure to high and low temperatures, darkness and 5% CO2. Planta 180, 61–73
Baylies, M.K., Bargiello, T.A., Jackson, F.R., Young, M.W. (1987) Changes in abundance or structure of the per gene product can alter the periodicity of the Drosophila clock. Nature 326, 390–392
Bollig, I.G., Wilkins, M.B. (1979) Inhibition of the rhythm of CO2 metabolism in Bryophyllum leaves by cycloheximide and dinitrophenol. Planta 145, 105–112.
Carter, P.J., Nimmo, H.G., Fewson, C.A., Wilkins, M.B. (1990) Bryophyllum fedtschenkoi protein phosphatase type 2A can dephosphorylate phosphoenolpyruvate carboxylase. FEBS Lett. 263, 233–236
Carter, P.J., Nimmo, H.G., Fewson, C.A., Wilkins, M.B. (1991) Circadian rhythms in the activity of a plant protein kinase. EMBO J. 10, 2063–2068
Carter, P.J., Wilkins, M.B., Nimmo, H.G., Fewson, C.A. (1995) Effects of temperature on the activity of phosphoenolpyruvate carboxylase and on the control of CO2 fixation in Bryophyllum fedtschenkoi. Planta 196, 377–382
Dunlap, J.C. (1990) Closely watched clocks: molecular analysis of circadian rhythms in Neurospora and Drosophila. Trends Genet. 6, 159–165
Friemert, V., Heininger, D., Kluge, M., Ziegler, H. (1988) Temperature effects on malic-acid efflux from the vacuoles and on the carboxylation pathways in Crassulacean-acid-metabolism plants. Planta 174, 453–461
Kluge, M., Klienchen, A., Galla, H-J. (1991) Temperature effects on crassulacean acid metabolism: EPR spectroscopic studies on the thermotropic phase behaviour of the tonoplast membranes in Kalanchoë daigremontiana. Bot. Acta 104, 355–360
McClung, C.R., Fox, B.A., Dunlap, J.C. (1987) The Neurospora clock gene frequency shares a sequence element with the Drosophila clock gene period. Nature 339, 558–562
Möllering, H. (1974) l(-)Malate determinations with MDH and GOT. In: Methods of enzymatic analysis, vol 3, pp. 1589–1593 Bergmeyer, H.U., ed. Academic Press New York
Nimmo, G.A., Nimmo, H.G., Fewson, C.A., Wilkins, M.B. (1984) Diurnal changes in the properties of phosphoenolpyruvate carboxylase in Bryophyllum leaves: a possible covalent modification. FEBS Lett. 178, 199–203
Nimmo, G.A., Nimmo, H.G., Hamilton, I.D., Fewson, C.A., Wilkins, M.B. (1986) Purification of the phosphorylated night form and dephosphorylated day form of phosphoenolpyruvate carboxylase from Bryophyllum fedtschenkoi. Biochem. J. 239, 213–220
Nimmo, G.A., Nimmo, H.G., Fewson, C.A., Wilkins, M.B. (1987) Persistent circadian rhythms in the phosphorylation state of phosphoenolpyruvate carboxylase from Bryophyllum fedtschenkoi leaves and in its sensitivity to inhibition by malate. Planta 170, 408–415
Osmond, C.B. (1978) Crassulacean acid metabolism: a curiosity in context. Annu. Rev. Plant Physiol. 29, 379–414
Wedding, R.T. (1989) Malic enzyme in higher plants. Plant Physiol. 90, 367–371
Wilkins, M.B. (1959) An endogenous rhythm in the rate of carbon dioxide output of Bryophyllum I. Some preliminary experiments. J. Exp. Bot. 10, 377–390
Wilkins, M.B. (1962) An endogenous rhythm in the rate of carbon dioxide output of Bryophyllum III. The effects of temperature on the phase and period of the rhythm. Proc. R. Soc. London 156, 220–241
Wilkins, M.B. (1983) The circadian rhythm of carbon-dioxide metabolism in Bryophyllum: the mechanism of phase-shift induction by thermal stimuli. Planta 157, 471–480
Wilkins, M.B. (1984) A rapid circadian rhythm of carbon-dioxide metabolism in Bryophyllum fedtschenkoi. Planta 161, 381–384
Wilkins, M.B. (1989) On the mechanism of phase control by light in the rhythm of carbon dioxide output in leaves of Bryophyllum fedtschenkoi. J. Exp. Bot. 40, 1315–1321
Wilkins, M.B. (1992) Circadian rhythms: their origin and control. New Phytol. 121, 347–375
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We thank the Agricultural and Food Research Council for financial support for this work.
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Carter, P.J., Wilkins, M.B., Nimmo, H.G. et al. The role of temperature in the regulation of the circadian rhythm of CO2 fixation in Bryophyllum fedtschenkoi . Planta 196, 381–386 (1995). https://doi.org/10.1007/BF00201399
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DOI: https://doi.org/10.1007/BF00201399