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Spinach leaf 70-kilodalton heat-shock cognate stabilizes bovine adrenal glucose-6-phosphate dehydrogenase in vitro without apparent stable binding

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Abstract

Spinach (Spinacia oleracea L.) leaf tissue 70-kilodalton heat-shock cognate was purified by ATP-agarose affinity and gel filtration. Gel filtration of the affinity-purified protein resolved it into three forms: monomer, dimer, and oligomer. In the absence of ATP, the majority of the heat-shock cognate existed as a monomeric form with lesser amounts of dimer and oligomer. Addition of 3 mM ATP to the purified protein, containing all three forms, converted the dimeric and monomeric forms to a high-molecular-weight complex. Removal of ATP from the complex by dialysis resulted in the reappearance of the dimeric and monomeric forms. Addition of ATP to the highly purified monomer had no effect on its gel-filtration migration. Neither purified monomeric or dimeric forms showed stable binding to denatured proteins; however, both forms of the purified heat-shock cognate were able to stabilize the enzymatic activity of bovine adrenal glucose-6-phosphate dehydrogenase over a 48-h period at 25° C. In addition, the activity of glucose-6-phosphate dehydrogenase in the presence of purified heat-shock cognate dimer or monomer could be rapidly decreased in an ATP-dependent fashion depending on the order of the substrate addition to the reaction mixture. Circular-dichroism studies indicated that addition of ATP to the spinach 70-kDa heat-shock cognate caused a conformation change from α-helical to a greater β-sheet content. How conformational character may influence the stabilizing activity of the heat-shock cognate in a mechanism which does not require stable peptide binding is discussed.

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Abbreviations

BiP:

immunoglobulin binding protein

BSA:

bovine serum albumin

CA:

carbonic anhydrase

CD:

circular dichroism

G6PDH:

glucose-6-phosphate dehydrogenase

HSP:

heat shock protein

HSP70:

70-kilodalton heat shock protein

HSC70:

70-kilodalton heat-shock cognate

MDH:

malate dehydrogenase

PVDF:

polyvinylidene difluoride

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Authors and Affiliations

  1. Plant Molecular and Cellular Biology Program, Department of Environmental Horticulture, Institute of Food and Agricultural Sciences, University of Florida, 32611-0512, Gainesville, FL, USA

    James V. Anderson & Charles L. Guy

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  1. James V. Anderson
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  2. Charles L. Guy
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Correspondence to Charles L. Guy.

Additional information

The authors wish to thank C. Kaye and R. Henry for their critical review and discussion of this paper. The antibody for tobacco BiP was the generous gift of J. Denecke. Financial support for this work was provided by the National Science Foundation DCB 9017625. This is Florida Agricultural Experiment Station Journal Series No. R-04110.

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Anderson, J.V., Guy, C.L. Spinach leaf 70-kilodalton heat-shock cognate stabilizes bovine adrenal glucose-6-phosphate dehydrogenase in vitro without apparent stable binding. Planta 196, 303–310 (1995). https://doi.org/10.1007/BF00201389

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