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Planta

, Volume 196, Issue 2, pp 284–287 | Cite as

Glycosyl-phosphatidylinositol-anchored proteins exist in the plasma membrane of Chlorella saccharophila (Krüger) Nadson: Plasma-membrane-bound nitrate reductase as an example

  • Christine Stöhr
  • Frank Schuler
  • Rudolf Tischner
Article

Abstract

Experiments with plasma-membrane vesicles were performed in order to identify the attachment of hydrophobic nitrate reductase at the plasma membrane of Chlorella saccharophila. The enzyme was successfully removed from the plasma membrane with phosphoinositol-specific phospholipase C, and showed cross-reactivity with a monoclonal antibody (clone aGPI-3) raised against the glycosyl-phosphatidylinositol (GPI) anchor of Trypanosoma variant surface protein. The enzyme was labelled in vivo by feeding [3H]ethanolamine to the cells and underwent an hydrophobicity shift after treatment with phosphoinositol-specific phospholipase C. The attachment of this form of nitrate reductase to the plasma membrane via a GPI anchor was demonstrated.

Key words

Chlorella Glycosyl-phosphatidylinositol anchor Nitrate reductase Plasma membrane 

Abbreviations

GPI

glycosyl-phosphatidylinositol

NR

nitratereductase

PI-PLC

phosphoinositol-specific phospholipase C

PMNR

Plasma-membrane-bound nitrate reductase

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Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • Christine Stöhr
    • 1
  • Frank Schuler
    • 2
  • Rudolf Tischner
    • 1
  1. 1.Pflanzenphysiologisches InstitutUniversität GöttingenGöttingenGermany
  2. 2.Physiologisch-Chemisches InstitutUniversität BonnBonnGermany

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