Summary
A sample of 450 sera from unrelated individuals from Southern Germany was examined by isoelectric focusing on polyacrylamide gels. Three common subtypes, TfC1, C2-1, and C2, were differentiated. In addition, the rare variants TfB1, B1-2, B2, D1, D1-2, D2, and D3 were observed. The frequencies of the Tf alleles in our sample were found to be: TfC1=0.8544, TfC2=0.1367, TfB1=0.0011, TfB1-2=0.0022, TfB2=0.0045, and TfD1=0.0011. Analysis of 73 parents with 73 children did not show deviations from the expected mode of inheritance. Modification of the method by addition of 0.01 M FeCl3 to the sera prior to examination did, however, reveal further variation and permitted the distinction of six subtypes, C1, C2-1, C2, C3, C3-1, and C3-2.
Zusammenfassung
Mit Hilfe der isoelektrischen Fokussierung in Polyacrylamidgelen (PAGIF) wurden 450 Proben von nicht verwandten Personen aus Süddeutschland untersucht. Es wurden drei häufige Untergruppen Tf C1, C2-1 und C2 differenziert, sowie die seltenen Varianten TfB1, B1-2, B2, D1, D1-2, D2 und D3 beobachtet. Die Allelfrequenzen in dieser Stichprobe betrugen: TfC1=0,8544, TfC2=0,1367, TfB1=0,0011, TfB1-2=0,0022, TfB2=0,0045, and TfD1=0,0011. Die Untersuchung von 73 Elternpaaren mit ihren Kindern ergab keine Abweichung vom angenommenen autosomal kodominanten Erbgang. Modifizierung der Methode durch Zusatz von 0,01 M FeCl3 zu den Seren vor der Auftrennung ließ weitere Variation erkennbar werden und erlaubte sechs häufige Untergruppen zu differenzieren, nämlich C1, C2-1, C2, C3, C3-1 und C3-2.
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This paper is dedicated to Fritz Hartmann, MD, Professor of Medicine, Hannover Medical School, FRG, on the occasion of his 60th birthday
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Weidinger, S., Schwarzfischer, F. & Cleve, H. Classification of transferrin (Tf) subtypes by isoelectric focusing. Z Rechtsmed 85, 255–261 (1980). https://doi.org/10.1007/BF00201287
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DOI: https://doi.org/10.1007/BF00201287