Summary
A series of examinations is presented for human origin identification and ABO blood grouping of doubtful minute human blood stains. A blood-stained thread (0.5 cm in length) was first tested to identify human origin by microprecipitation method and then the ABO blood type was determined by both a modified absorption-elution test and a modified mixed agglutination. In the continuous tests, the maximum limits of positive reactions of the microprecipitation method, the modified absorption-elution test, and the modified mixed agglutination were 1:640, 1:160, and 1:2,560 diluted blood, respectively. A and B agglutinogens were more sensitively determined than H agglutinogen. Hemagglutinogens of blood stains on cotton threads were more easily detected than those of polyester ones.
Zusammenfassung
Es werden drei kontinuierliche Verfahren zur Identifizierung von menschlichem Blut und zur Bestimmung der ABO-Blutgruppen an einem verdächtigen winzigen Blutfleck (ein Faden mit einer Länge von 0,5 cm) beschrieben. Nach der Menschenblutbestimmung des verdächtigen Blutflecks mittels Mikropräzipitationsmethode wurden die ABO-Blutgruppen durch eine modifizierte Absorptions-Elutions-Methode bestimmt, und schließlich wurde eine verbesserte Mischzellagglutination zur Blutgruppenbestimmung am verdächtigen Blutfleck ausgeführt. Die maximalen Grenzen der positiven Reaktionen lagen bei folgenden Blutverdünnungen: (1) Mikropräzipitationsmethode 1:640; (2) modifizierte Absorptions-Elutions-Methode 1:160 und (3) verbesserte Mischzellagglutination 1:2560. Die Empfindlichkeit der Feststellung von A- und B-Agglutinogen auf dem Blutfleck war höher als die von H-Agglutinogen. Der Nachweis von Hämagglutinogenen des Blutflecks wurde auf dem Baumwollfaden leichter erbracht als bei dem Blutfleck auf dem Polyesterfaden.
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Tokiwa, K. A sequence of tests of minute human blood stains for human origin identification and ABO blood grouping. Z Rechtsmed 97, 157–164 (1986). https://doi.org/10.1007/BF00201238
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DOI: https://doi.org/10.1007/BF00201238
Key words
- Forensic serial examination
- Species identification, blood stains
- ABO blood grouping, blood stains
- Microprecipitation method, absorption elution, mixed agglutination
- Blood stains