Summary
Optical isomers (d and l) and racemic compounds (dl) of methamphetamine (MAMP) and amphetamine (AMP), and biologic materials including those substances, could be analyzed by high performance liquid chromatography. Examining the temperature for the analysis, 40°C was the optimal condition in the reproducibility of separated MAMP-isomers. The reproducibility at the temperature did not vary significantly. The measured values of optical isomers were 0.116±0.012, 1.082±0.070 and 8.984±0.136 for the mixing ratios (l/d) of 0.111, 1.000, and 9.000, respectively. The detection limit for both d- and l-isomers was 25 ng.
The analytic result of hair specimens from two stimulant abusers by the present method indicates that they contained only d-MAMP and d-AMP, which is believed to have the strongest pharmacologic effect among the optical isomers of MAMP. The coefficient of variation in the analysis of five replicate standards, prepared by adding 1,000 ng each of racemate MAMP and AMP to hair, was less than 4%. The measured value against l/d=1.000 was 1.040±0.040 in MAMP and 0.980±0.030 in AMP. The detection limit for both racemate MAMP and AMP accumulated in hair was 250 ng.
The analysis of the optical isomers by our method would contribute to identifying the smuggling routes or the illicit method.
Zusammenfassung
Es wurde eine neue Methode zur Trennung von optisch aktiven Isomeren (d und l) und der racemischen Verbindung (dl) von Metamphetamin (MAMP) und Amphetamin (AMP) mittels Hochleistungsflüssigkeitschromatographie durch die UV-Methode ausgearbeitet. Wir fanden, daß 40°C in der Auflösung und der Reproduzierbarkeit der MAMP-Isomeren am besten waren. Meßwerte von Isomeren waren 0.116±0.012, 1.082±0.070 und 8.984±0.136 gegenüber den Mischungsverhältnissen (l/d) von 0.111, 1.000 und 9.000. Die Nachweisgrenze von d- und l-Isomeren war jeweils 25 ng.
Die Analysenergebnisse menschlicher Haare von zwei Stimulationsmittelkonsumenten zeigten mittels der oben genannten Methode, daß die Haare nur d-MAMP und d-AMP enthielten, welche unter den Isomeren die stärksten pharmakologischen Wirkungen haben. Die Streuung der Analysen von fünf Standard-Materialien, nach Zusatz von 1,000 ng jeder racemischen MAMP und AMP zu menschlichen normalen Kopfhaaren, war weniger als 4%; die Meßwerte von Isomeren gegenüber den Mischungsverhältnissen l/d=1.000, waren 1.040±0.040 bei MAMP und 0.980±0.030 bei AMP. Die Nachweisgrenze der racemischen Verbindung von MAMP und AMP, die in Haaren angehäuft sind, war jeweils 250 ng.
Die Analyse der Isomeren von MAMP und AMP durch unsere Methode würde der Aufdeckung von Schmuggelungen und -methoden dienen.
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Nagai, T., Kamiyama, S. Forensic toxicologic analysis of methamphetamine and amphetamine optical isomers by high performance liquid chromatography. Z Rechtsmed 101, 151–159 (1988). https://doi.org/10.1007/BF00201110
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DOI: https://doi.org/10.1007/BF00201110