Summary
A simple qualitative method for identification of seminal stains based on a high activity of γ-glutamyltransferase (γ-GTP) in human semen is described. It employs the release of α-naphthylamine from N-γ-glutamyl-α-naphthylamide by the γ-GTP action; α-naphthylamine couples with Fast Garnet GBC salt to produce a strong brownish-red color. The data on its simplicity, specificity, and stability show that the present method is suitable for medicolegal examination of seminal stains as a preliminary test.
Zusammenfassung
Es wird eine einfache, qualitative Methode zur Identifizierung von Spermaspuren beschrieben, die auf der hohen γ-Glutamyltransferase-Aktivität (γ-GTP) des menschlichen Sperma basiert. Dabei wird die Abspaltung von α-Naphthylamin von N-γ-Glutamyl-α-naphthylamid durch das Enzym benutzt, wobei α-Naphthylamin mit Fast Garnet GBC-Salz gekoppelt eine tief braunrote Farbe bildet. Die Ergebnisse der Untersuchung bestätigen die Einfachheit, Spezifität und Stabilität dieser Methode als Vortest zur forensischen Untersuchung von Spermaspuren.
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References
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Suzuki, O., Oya, M., Kido, A. et al. γ-Glutamyltransferase test as a new tool for identification of seminal stains. Z Rechtsmed 86, 35–39 (1980). https://doi.org/10.1007/BF00200976
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DOI: https://doi.org/10.1007/BF00200976
Key words
- γ-Glutamyltransferase, identification of semen
- Seminal stains, identification by γ-glutamyltransferase