Summary
Glycolipids extracted from groups A, B, and 0 erythrocytes were developed on thin-layer plates; their AB0 blood group antigenicities were detected by immunostaining method using avidin-biotin-complex (ABC). Among series of glycolipids of different flow rates, antigen-specific staining was observed in five bands from group A1 erythrocytes, four bands from group B, and two bands from group O. Monoclonal anti-A, -B, and -H antibodies specifically stained glycolipids from A1, B, and 0 erythrocytes, respectively.
AB0 blood grouping was possible from 5 g of epidural heat hematoma of a charred body by this method. ABC immunostaining on thin-layer chromatography is a useful and reliable method for AB0 blood grouping in forensic practice.
Zusammenfassung
Glycolipide, welche von Erythrozyten der Blutgruppen A, B und 0 extrahiert wurden, wurden mittels der Dünnschichtchromatographie nachgewiesen; ihre AB0-Blutgruppenprägung wurde mit Hilfe der Immunfärbungsmethode unter Verwendung von Avidin-Biotin-Complexen nachgewiesen. Unter den Serien von Glycolipiden mit verschiedenen Fließgeschwindigkeiten wurden antigenspezifische Färbungen in fünf Banden von Erythrozyten der Blutgruppe A festgestellt, in vier Banden von Erythrozyten der Blutgruppe B und in 2 der Blutgruppe 0. Monoklonale Anti-A, B- und H-Antikörper färbten spezifisch Glycolipide der jeweiligen Erythrozyten.
Im Falle einer verkohlten Leiche war die AB0-Blutgruppenbestimmung aus 5 g eines epiduraden Hitze-Hämatoms möglich. Die Immunfärbung mit Hilfe des Avidin-Biotin-Complexes auf Dünnschichtchromatographie-Platten ist eine nützliche und zuverlässige Methode für die AB0-Blutgruppenbestimmung in der forensischen Praxis.
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Supported in part by a scientific grant from the Ministry of Education, Science, and Culture, Japan
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Ota, M., Fukushima, H., Yonemura, I. et al. Detection of AB0 blood group-active glycolipids extracted from red cell membrane and heat hematoma using TLC-immunostaining. Z Rechtsmed 100, 215–221 (1988). https://doi.org/10.1007/BF00200762
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DOI: https://doi.org/10.1007/BF00200762