Summary
Soluble tumor extracts were isolated from a UV light-induced mouse sarcoma by 3 M KCl extraction followed by 2 M (NH4)2SO4 precipitation. The precipitates dissolved in phosphate buffered saline (PBS) produced transplantation resistance against syngeneic cell inocula of the tumor at a restricted dose range. While 1.0–1.2 mg of protein per mouse were effective, double this dose was not. An extract prepared from ascitic fluid of tumor-bearing mice showed effects similar to one prepared from tumor cells.
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Pasternak, L., Pasternak, G. & Karsten, U. Immunogenicity of soluble extracts from a UV light-induced mouse sarcoma. Cancer Immunol Immunother 3, 273–275 (1978). https://doi.org/10.1007/BF00200081
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DOI: https://doi.org/10.1007/BF00200081