Summary
Radiation-attenuated, aminoazo dye-induced rat hepatoma cells were employed as a standard immunogen in tests evaluating the optimal conditions for the demonstration of resistance to subcutaneous challenge with viable tumour cells. These tests revealed that maximal sensitivity for the detection of the immunogenicity of γ-irradiated cells was obtained using multiple immunisations via the intraperitoneal route rather than the subcutaneous route. When 3 M KCl extracts of tumours (as soluble tumour antigens) were evaluated for the induction of specific immunoprotection under comparable conditions, weak resistance to tumour was demonstrable, but only within a restricted dose range of the antigen preparation. No significant effects upon tumour growth were observed in rats pretreated with fractions of tumour-bearer serum.
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Price, M.R., Preston, V.E., Robins, R.A. et al. Induction of immunity to chemically-induced rat tumours by cellular or soluble antigens. Cancer Immunol Immunother 3, 247–252 (1978). https://doi.org/10.1007/BF00200078
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DOI: https://doi.org/10.1007/BF00200078