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Comparative studies between in vitro and in vivo effects of human beta-interferon on natural killer activity and its relevance to immunochemotherapy

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Summary

A good correlation was found between in vivo and in vitro responses of peripheral MNC from breast cancer patients and the NK boosting effect of human βIFN. In vitro immunochemotherapy studies showed that marked antitumor effects were obtained against cultured cancer cells when a widely used chemotherapeutic agent such as 5-FU was combined with nonsensitized spontaneously cytolytic MNC, preactivated in vitro with βIFN. These results suggest that the in vitro susceptibility assay of MNC to IFNs could be used for predicting favorable responses to immunochemotherapy regimens employing IFNs as immunomodulating agents.

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Authors and Affiliations

  1. Institute of Experimental Medicine, National Council of Research (CNR), Rome, Italy

    Stefania D'Atri, Maria Pia Fuggetta & Lucio Tentori

  2. Department of Experimental Medicine and Biochemical Sciences, School of Medicine, II University of Rome, Via O. Raimondo, 1-00173, Rome, Italy

    Gabriella Giganti, Mario Roselli, Corrado Nunziata, Simonetta Pastore, Enzo Bonmassar & Liana De Vecchis

Authors
  1. Stefania D'Atri
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  2. Maria Pia Fuggetta
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  3. Gabriella Giganti
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  4. Lucio Tentori
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  5. Mario Roselli
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  6. Corrado Nunziata
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  7. Simonetta Pastore
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  8. Enzo Bonmassar
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  9. Liana De Vecchis
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Additional information

Abbreviations used: βIFN, beta-interferon; CM, complete medium; CTX, cyclophosphamide; E:T ratio, effector target ratio; EDTA, ethylenediaminetetraacetic acid; FCS, fetal calf serum; 5-FU, 5-fluorouracil; γIFN, gamma-interferon; Hepes, N-2-hydroxyethylpiperazine-N-2-ethanesulphonic acid IFNs, interferons; KC, killed cells; LDIF, low doses beta-interferon; LU, lytic unit; MAT macrotest; MIT, microtest; MNC, mononuclear cells; MNC/IFN, βIFN-pretreated MNC; MTX, methotrexate; NB, no boosting; NK, natural killer; NKA, NK activity; NR, natural resistance; PM, positive modulation; SE, standard error; WM, washing medium

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D'Atri, S., Fuggetta, M.P., Giganti, G. et al. Comparative studies between in vitro and in vivo effects of human beta-interferon on natural killer activity and its relevance to immunochemotherapy. Cancer Immunol Immunother 27, 163–170 (1988). https://doi.org/10.1007/BF00200022

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  • DOI: https://doi.org/10.1007/BF00200022

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