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Distribution of phytochrome and chlorophyll-a/b-binding-protein mRNAs in etiolated Avena seedlings

Planta volume 187, pages 532–536 (1992)Cite this article

Abstract

In 4-d-old dark-grown oat (Avena sativa L.) seedlings, the majority of the type-I-phytochrome (phyA) mRNA was found within 10 mm of the tip of the coleoptile sheath and in the mesocotyl node; almost none was detected in the enclosed primary leaf. In contrast, chlorophyll-a/b-binding-protein (cab) mRNAs were found almost exclusively in the enclosed primary leaf and were barely detectable in total-RNA samples from the coleoptile sheath or mesocotyl node of red-light-treated etiolated seedlings. Separated, dark-grown primary leaves responded to a red-light treatment by increasing cab-mRNA abundance in the absence of the coleoptile sheath or mesocotyl node tissues.

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Abbreviations

cab :

gene for chlorophyll-a/b-binding protein

kb:

kilobase

phyA :

gene for type-I-phytochrome protein

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Affiliations

  1. Department of Botany, Iowa State University, 50011, Ames, IA, USA

    Kevin A. Seeley & James T. Colbert

Authors
  1. Kevin A. Seeley
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  2. James T. Colbert
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Additional information

We are grateful to the members of the laboratory Dave Higgs, Theresa Tirimanne, Dr. Dennis Byrne, Bruce Held, Linda Barnes, Dr. Isaac John, and Iffat Rahim, for their helpful discussions and critical review. This work was supported by USDA grant No. 88-37261-4196 and No. 91-37304-6397, the Iowa State University Biotechnology Program, and the Molecular, Cellular, and Developmental Biology Program.

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Seeley, K.A., Colbert, J.T. Distribution of phytochrome and chlorophyll-a/b-binding-protein mRNAs in etiolated Avena seedlings. Planta 187, 532–536 (1992). https://doi.org/10.1007/BF00199973

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  • DOI: https://doi.org/10.1007/BF00199973

Key words

  • Avena (phytochrome)
  • Chlorophyll-a/bbinding protein
  • Light regulation
  • mRNA localization
  • Phytochrome