Abstract
In 4-d-old dark-grown oat (Avena sativa L.) seedlings, the majority of the type-I-phytochrome (phyA) mRNA was found within 10 mm of the tip of the coleoptile sheath and in the mesocotyl node; almost none was detected in the enclosed primary leaf. In contrast, chlorophyll-a/b-binding-protein (cab) mRNAs were found almost exclusively in the enclosed primary leaf and were barely detectable in total-RNA samples from the coleoptile sheath or mesocotyl node of red-light-treated etiolated seedlings. Separated, dark-grown primary leaves responded to a red-light treatment by increasing cab-mRNA abundance in the absence of the coleoptile sheath or mesocotyl node tissues.
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Abbreviations
- cab :
-
gene for chlorophyll-a/b-binding protein
- kb:
-
kilobase
- phyA :
-
gene for type-I-phytochrome protein
References
Briggs, W.R., Siegelman, H.W. (1965) Distribution of phytochrome in etiolated seedlings. Plant Physiol. 40, 934–941
Bruce, W.B., Deng, X.-W., Quail, P.H. (1991) A negatively acting DNA sequence element mediates phytochrome-directed repression of phyA gene transcription. EMBO J. 10, 3015–3024
Colbert, J.T. (1988) Molecular biology of phytochrome. Plant Cell Environ. 11, 305–318
Colbert, J.T. (1991) Regulation of type I phytochrome mRNA abundance. Physiol. Plant. 82, 327–332
Colbert, J.T., Hershey, H.P., Quail, P.H. (1983) Autoregulatory control of translatable phytochrome mRNA levels. Proc. Natl. Acad. Sci. USA 80, 2248–2252
Edwards, C.L., Colbert, J.T. (1990) Regulation of phytochrome mRNA abundance in green oat leaves. Plant Cell Environ. 13, 813–819
Johnson, E.M. Pao, L.I., Feldman, L.J. (1991) Regulation of phytochrome message abundance in root caps of maize. Plant Physiol. 96, 544–550
Kuhlemeier, C., Green, P.J., Chua, N.-H. (1987) Regulation of gene expression in higher plants. Annu. Rev. Plant Physiol. 38, 221–257
Kondo, N., Inoue, Y., Shibata, K. (1973) Phytochrome distribution in Avena seedlings measured by scanning a single seedling. Plant Sci. Let. 1, 165–168
Lissemore, J.L., Quail, P.H. (1988) Rapid transcriptional regulation by phytochrome of the genes for phytochrome and chlorophyll a/b-binding protein in Avena sativa. Mol. Cell. Biol. 8, 4840–4850
Pratt, L.H. (1986) Localization within the plant. In: Photomorphogenesis in plants, pp. 61–81, Kendrick, R.E., Kronenburg, G.H.M., eds. Martinus Nijhoff, Amsterdam
Pratt, L.H., Coleman, R.A. (1974) Phytochrome distribution in etiolated grass seedlings as assayed by an indirect antibodylabelling method. Am. J. Bot. 61 195–202
Schaer, J.A., Mandoli, D.F., Briggs, W.R. (1983) Phytochrome-mediated cellular photomorphogenesis. Plant Physiol. 72, 706–712
Seeley, K.A., Byrne, D., Colbert, J.T. (1992) Red light-independent instability of oat phytochrome mRNA in vivo. Plant Cell 4, 29–38
Shanklin, J., Jabben, M., Vierstra, R.D. (1987) Red light-induced formation of ubiquitin-phytochrome conjugates: Identification of possible intermediates of phytochrome degradation. Proc. Natl. Acad. Sci. USA 81, 359–363
Sharrock, R.A., Quail. P.H. (1989) Novel phytochrome sequences in Arabidopsis thaliana: Structure, evolution, and differential expression of a plant regulatory photoreceptor family. Genes Devel. 3, 1745–1757
Silverthorn, J., Tobin, E.M. (1987) Phytochrome regulation of nuclear gene expression. BioEssays 7, 18–23
Tomizawa K.I., Matasuji, E., Ishii, K. (1991) Distribution of type I phytochrome (phyA) RNA1 and RNA2 in etiolated pea seedlings. J. Photochem. Photobiol. 11, 163–172
Wadsworth, G.J., Redinbaugh, M.G., Scandalios, J.G. (1988) A procedure for the small-scale isolation of plant RNA suitable for RNA blot analysis. Anal. Biochem. 172, 279–283
Watson, J.C. (1989) Photoregulation of gene expression in plants. In: Plant biotechnology, pp. 161–205, Kung, S., Arntzen, C.J. eds. Butterworths, Boston
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We are grateful to the members of the laboratory Dave Higgs, Theresa Tirimanne, Dr. Dennis Byrne, Bruce Held, Linda Barnes, Dr. Isaac John, and Iffat Rahim, for their helpful discussions and critical review. This work was supported by USDA grant No. 88-37261-4196 and No. 91-37304-6397, the Iowa State University Biotechnology Program, and the Molecular, Cellular, and Developmental Biology Program.
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Seeley, K.A., Colbert, J.T. Distribution of phytochrome and chlorophyll-a/b-binding-protein mRNAs in etiolated Avena seedlings. Planta 187, 532–536 (1992). https://doi.org/10.1007/BF00199973
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DOI: https://doi.org/10.1007/BF00199973