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Heterogeneity of primary and metastatic human malignant melanoma as detected with monoclonal mntibodies in cryostat sections of biopsies

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Summary

Monoclonal antibodies were generated against established melanoma cell lines and characterized by their reactivity with various sublines. The antibodies selected for their reaction with melanoma-associated antigens were tested on cryostat sections of melanoma tissue from various stages and on other tumors. The reactivity with normal tissues was also determined. Of 30 antibodies reacting with melanoma cell lines 11 did not react with melanoma biopsies. Of the remaining 19 antibodies nine displayed broad cross-reactivity with normal cells and structures and other benign or malignant tumor cells. Among the remaining antibodies five types were defined that detected antigens (nevocellular I, nevocellular II, neural, endothelial, basal cell) found on certain normal tissues and structures and on certain tumor phenotypes. Even though there seems to be a tendency for some antigens to be preferentially associated with certain stages of melanoma, it has not yet been possible to establish any clear-cut correlation between the expression of one of the differentiation antigens and a particular stage or malignancy potential of melanoma.

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Authors and Affiliations

  1. Fachklinik Hornheide, Münster-Handorf, Federal Republic of Germany

    Ludwig Suter

  2. Abteilung für Experimentelle Dermatologie, Universitäts-Hautklinik, Von Esmarch-Strasse 5b, D-4400, Münster, Federal Republic of Germany

    Eva-Bettina Bröcker, Josef Brüggen & Clemens Sorg

  3. Pathologisch Laboratorium, Universitair Medisch Centrum, Leiden, The Netherlands

    Dirk J. Ruiter

Authors
  1. Ludwig Suter
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  2. Eva-Bettina Bröcker
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  3. Josef Brüggen
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  4. Dirk J. Ruiter
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  5. Clemens Sorg
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Suter, L., Bröcker, EB., Brüggen, J. et al. Heterogeneity of primary and metastatic human malignant melanoma as detected with monoclonal mntibodies in cryostat sections of biopsies. Cancer Immunol Immunother 16, 53–58 (1983). https://doi.org/10.1007/BF00199906

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  • DOI: https://doi.org/10.1007/BF00199906

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