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Tumor-derived interleukin-2-dependent lymphocytes in adoptive immunotherapy of lung cancer

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Summary

A trial of adoptive immunotherapy was performed in which long-term cultured, interleukin-2 (IL2)-dependent T-lymphocytes were administered to patients with metastatic adenocarcinoma of the lung. Lymphocytes were isolated from explants of cancer tissues that were cultured in medium with recombinant IL-2. These T-cells expressed surface markers of activation, and killed a broad panel of tumor targets. Intravenously injected 111indium-labeled T-cell blasts distributed primarily to lungs, liver, and spleen. Despite a paucity of infused lymphocytes detected by external imaging at sites of tumor, five of seven patients showed reduction of their cancers. However, in no case was greater than 50% reduction of total tumor burden achieved. Evidence of increased delayed cutaneous hypersensitivity to protein antigens was observed in three patients following therapy. We conclude that long-term cultured tumor-derived T-cells can be transferred safely into humans and that these cells may be capable of enhancing immune responses and mediating tumor reduction in vivo.

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Authors and Affiliations

  1. Medicine, Radiology and Pathology, Departments of the Massachusetts General Hospital and the Harvard Medical School, Boston, Massachusetts, USA

    Richard L. Kradin, Lenora A. Boyle, Frederic I. Preffer, Ronald J. Callahan, Martha Barlai-Kovach, H. William Strauss, Steven Dubinett & James T. Kurnick

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  1. Richard L. Kradin
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  2. Lenora A. Boyle
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  3. Frederic I. Preffer
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  4. Ronald J. Callahan
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  5. Martha Barlai-Kovach
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  6. H. William Strauss
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  7. Steven Dubinett
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  8. James T. Kurnick
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Kradin, R.L., Boyle, L.A., Preffer, F.I. et al. Tumor-derived interleukin-2-dependent lymphocytes in adoptive immunotherapy of lung cancer. Cancer Immunol Immunother 24, 76–85 (1987). https://doi.org/10.1007/BF00199837

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  • DOI: https://doi.org/10.1007/BF00199837

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