Abstract
The patterns of lipid-body proteins prepared at different stages of germination from seedlings of anise (Pimpinella ansium L.) were investigated. During the stage of fat mobilization, a set of proteins is synthesized de novo and transferred to existing lipid bodies. Analysis of the protein constituents, detected either by protein staining, concanavalin-A/peroxidase staining or immunoreaction on Western blots showed three distinct protein species: a lipoxygenase firmly integrated into the lipid bodies, a glycoprotein gp50 only extractable from the lipid bodies by treatment with sodium dodecyl sulfate and a glycoprotein gp49 solubilized by diethyl-ether treatment of lipid bodies. Both the lipoxygenase immunodetected on Western blots and gp49 stained with concanavalin-A/ peroxidase conjugate occurred transiently in the lipidbody fraction, reaching maximum concentrations between days 6 and 13 of germination. This behavior was in contrast to the decreasing level of the 18.4-kDa oleosin already present at the beginning of germination.
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Abbreviations
- gp:
-
glycoprotein
- PAGE:
-
polyacrylamide gel electrophoresis
- SDS:
-
sodium dodecyl sulfate
References
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We would like to thank Christiane Bernshausen and Willi Jonghaus for kind technical assistance. This research was supported by a grant from the Deutsche Forschungsgemeinschaft. I.F. is a recipient of the Friedrich Ebert-Stiftung.
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Radetzky, R., Feussner, I., Theimer, R.R. et al. Transient occurrence of lipoxygenase and glycoprotein gp49 in lipid bodies during fat mobilization in anise seedlings. Planta 191, 166–172 (1993). https://doi.org/10.1007/BF00199746
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DOI: https://doi.org/10.1007/BF00199746