Abstract
The physiological and biochemical consequences of treating Le (tall) and le (dwarf) pea seedlings with varying quantities of the gibberellins [3H]GA20 and GA1 have been investigated. Although the percentage uptake of these compounds from the site of application on the 3∘ stipules was low and most of the applied GA remained unmetabolised in situ, the quantitative relationship between GA translocation and GA dosage was found to be linear for GA1 but saturating for GA20. The movement of the GAs and their subsequently produced metabolites was mainly acropetal. They accumulated in greatest quantity in the apical extremities of the shoot. Overall, the extent to which GA20 was metabolished in le seedlings was considerably less than in Le pea seedlings. Although all le tissues contained significantly less [3H]GA1 than their Le counterparts, phenotypic effects of the le mutation were apparent only on internode and tendril development. Increased tissue growth, consequent upon GA treatment, was also apparent only in the internodes and tendrils of le plants. For internodes, GA1 content determined the mid-logarithmic-phase growth rate and, consequently, final length. For tendrils, GA20 rather than GA1 may be the primary stimulatory agent.
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Abbreviations
- GA:
-
gibberellin
- HPLC:
-
high-performance liquid chromatography
- 1∘–6∘:
-
consecutive developmental numbering system for plant tissues/organs as shown in Fig. 1
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The author gratefully acknowledges financial support from Imperial Chemical Industries, Plant Protection, Jealott's Hill, Bracknell, Berks., UK and the Science and Engineering Research Council.
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Smith, V.A. Gibberellin translocation in Pisum sativum L.. Planta 191, 158–165 (1993). https://doi.org/10.1007/BF00199745
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DOI: https://doi.org/10.1007/BF00199745