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Distribution of intact and Fab1 2 fragments of anti-human chorionic gonadotrophin antibodies in nude mice bearing human choriocarcinoma xenografts

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Summary

A direct comparative study of the distribution of intact and Fab1 2 fragments of mouse monoclonal anti-human chorionic gonadotrophin antibody, W14A, in nude mice bearing a human choriocarcinoma xenograft, indicates that the tumour:blood ratio is generally improved with fragments. Some parameters which may determine the distribution are outlined.

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References

  1. Arend WP, Teller DC, Mannik M (1972) Molecular composition and sedimentation characteristics of soluble ag — ab complexes. Biochemistry 11:4063

    Google Scholar 

  2. Begent RHJ, Bagshawe KD (1985) Clinical applications of radioimmunolocalisation. In: Baldwin RW (ed) Monoclonal antibodies for tumour detection and drug targeting. Academic Press, London (in press)

    Google Scholar 

  3. Brennan FM, Grace SA, Elson CJ (1983) Preparation of covalent IgG complexes of defined size and their clearance from the circulation of mice. J Immunol Meth 56:149

    Google Scholar 

  4. Buchegger F, Haskell CM, Schreyer M, Scazziga BR, Randir S, Carrel S, Mach J-P (1983) Radiolabelled fragments of monoclonal antibodies against carcinoembryonic antigen for localisation of human colon carcinoma grafted into nude mice. J Exp Med 158:413

    Google Scholar 

  5. Burchiel SW, Burke D, Breslow K, Austin R, Reed K, Rhodes B (1982) Biodistribution of radiolabelled antibodies and their fragments: an immunopharmacologic approach to in vivo tumour imaging. Proc West Pharmacol Soc 25:405

    Google Scholar 

  6. Cavalieri RR, Rapaport B (1977) Impaired peripheral conversion of thyroxine to triiodothyronine. Ann Rev Med 28:57

    Google Scholar 

  7. Colcher D, Zalutsky M, Kaplan W, Kufe D, Austin F, Schlom J (1983) Radioimmunolocalisation of human mammary tumours in athymic mice by a monoclonal antibody. Cancer Res 43:736

    Google Scholar 

  8. Ellman GL (1959) Tissue sulfhydryl groups. Arch Biochem Biophys 82:70

    Google Scholar 

  9. Folkers K, Shin S, Kappel M (1983) Structurally restricted conjugates of the subunit of human chorionic gonadotrophin to produce antisera. Biochem Inter 7,6:761

    Google Scholar 

  10. Herlyn D, Powe J, Alavi A, Mattis JA, Herlyn M, Ernst C, Vaum R, Koprowski H (1983) Radioimmunodetection of human tumour xenografts by monoclonal antibodies. Cancer Res 43:2731

    Google Scholar 

  11. Johnson PM, Brown PM (1981) Review Article: Fc receptors in the human placenta. Placenta 2:355

    Google Scholar 

  12. Mannik M, Arend WP, Hall AP, Gilliland BC (1971) Studies on antigen-ab complexes: elimination of soluble complexes from rabbit circulation. J Exp Med 133:713

    Google Scholar 

  13. Morgan FJ, Canfield RE (1971) Nature of the subunits of human chorionic gonadotrophin. Endocrinology 88:1045

    Google Scholar 

  14. Morgan FJ, Birken S, Canfield RE (1975) The amino acid sequence of human chorionic gonadotrophin. J Biol Chem 250:5247

    Google Scholar 

  15. Mason AW, Williams AF (1980) The kinetics of antibody binding to membrane antigens in solution and at the cell surface. Biochem J 187:1

    Google Scholar 

  16. Nisonoff A, Hopper JE, Spring SB (1975) The antibody molecule. Academic Press, New York, p 235

    Google Scholar 

  17. Nisonoff A, Hopper JE, Spring SB (eds) (1975) The Antibody Molecule. Academic Press, New York p 325

    Google Scholar 

  18. Ogston AG, Preston BN, Wells JD (1973) The transport of compact particles through solutions of chain-polymers. Proc Soc Lond A 333:297

    Google Scholar 

  19. Poste G (1983) Liposome targeting in vivo: problems and opportunities. Biol Cell 47:19

    Google Scholar 

  20. Quinones J, Mizejewski G, Beierwaltes WH (1971) Choriocarcinoma scanning using radiolabelled antibody to chorionic gonadotrophin. J Nucl Med 12,2:69

    Google Scholar 

  21. Scherphof G (1980) In Gregoriades G, Allison AC (eds), Liposomes in biological systems. John Wiley & Co, New York, p 179

    Google Scholar 

  22. Searle F, Boden J, Lewis JCM, Bagshawe KD (1981) A human choriocarcinoma xenograft in nude mice; a model for the study of antibody localisation. Br J Cancer 44:137

    Google Scholar 

  23. Searle F, Partridge CS, Kardana A, Green AJ, Buckley RG, Begent RHJ, Rawlins GA (1984) Preparation and properties of a mouse monoclonal antibody (W14A) to human chorionic gonadotrophin. Int J Cancer 33:429

    Google Scholar 

  24. Sedlacek HH, Gronski P, Hofstaetter T, Kanzy EJ, Schorlemmer HU, Seiter FR (1983) The biological properties of immunoglobulin G and its split products [F(ab1)2 and Fab]. Klin Wochenschr 61:723

    Google Scholar 

  25. Silverstein SC, Steinman RM, Cohn ZA (1977) Endocytosis. Ann Rev Biochem 46:669

    Google Scholar 

  26. Tomlinson E, McVie JG (1983) New directions in cancer chemotherapy 2. Targeting with microspheres. Pharmacy Inter 281

  27. Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proc Natl Acad Sci USA 76:4350

    CAS  PubMed  Google Scholar 

  28. Turner MW (1981) In: Glynn LE, Steward MW (eds) Structure and function of antibodies J Wiley & Sons, New York, p 38

    Google Scholar 

  29. Vaupel P, Muller-Kleiser W (1983) Interstitieller Raum und Mikromilieu in malignen tumoren. Mikrozirk Forsch Klin 20:78

    Google Scholar 

  30. Wataya Y, Santi DV (1975) Thymidylate synthetase catalysed dehalogenation of 5-bromo and 5-iodo-21-deoxyuridylate. Biochem Biophys Res Commun 67:818

    Google Scholar 

  31. Widder KJ, Senyei AE, Sears B (1982) Experimental methods in cancer therapeutics. J Pharm Sci 71:379

    Google Scholar 

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Authors and Affiliations

  1. Cancer Research Campaign Laboratories, Department of Medical Oncology, Charing Cross Hospital, W6 8RF, London, UK

    Frances Searle, Theresa Adam & Joan A. Boden

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  1. Frances Searle
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  2. Theresa Adam
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  3. Joan A. Boden
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Searle, F., Adam, T. & Boden, J.A. Distribution of intact and Fab1 2 fragments of anti-human chorionic gonadotrophin antibodies in nude mice bearing human choriocarcinoma xenografts. Cancer Immunol Immunother 21, 205–208 (1986). https://doi.org/10.1007/BF00199363

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  • DOI: https://doi.org/10.1007/BF00199363

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