Summary
The reactivity with monoclonal antibodies (MoAbs) specific for myelomonocytic cells and the expression of a particular esterase isoenzyme were analyzed in 159 cases of acute myeloid leukemias. The incidence of positivity of 16 MoAbs (MCS-2, MCS-1, OKM1, My-1, Leu-M1, Leu-M3, CA-2-38, MY4, MY7, MY8, MY9, VIM-D2, VIM-D5, Mo1, Mo2, 63D3) was studied using the indirect immunofluorescence technique. A carboxylic esterase isoenzyme which can be inhibited completely and selectively by sodium fluoride (NaF) was demonstrated by isoelectric focusing on horizontal polyacrylamide gels. This NaF-sensitive isoenzyme indicated the monocytic origin of the blast cells as it is specific for this cell lineage. Prior to the immunological-isoenzymatic analysis all cases were categorized into two subtypes according to morphological criteria of the FAB classification system: 147 cases of AML (FAB M1-3) and 12 cases of AMMoL/AMoL (FAB M4/5). However, 15 out of 147 cases of AML expressed the NaF-sensitive isoenzyme and were therefore assigned to the group AMMoL/AMoL. Likewise, 1 case, diagnosed morphologically as AMMoL, was negative for this marker isoenzyme and was assigned to the other leukemia subtype. The incidence of reactivity varied widely for the MoAbs tested regarding the overall results on all cases and the positivity on cases of either AML or AMMoL/AMoL. The MoAbs were grouped into four classes depending on the pattern of reactivity with myeloblastic or monoblastic or both subtypes of acute myeloid leukemia. The MoAbs MCS-2, MY7, Leu-M1, and MY9 detected the vast majority of cases with either myelocytic or monocytic involvement (group-I: “pan-myelomonocytic” reactivity). The MoAbs MCS-1, OKM1, VIM-D5, and Mo1 showed a predominance in their staining pattern for monocytic variants, but were also positive on a substantial percentage of nonmonocytic cases (group-II: predominantly reactive with monocytic, but also myelocytic cases). The MoAbs Leu-M3, MY4, VIM-D2, Mo2, and MY8 reacted with the large majority of AMMoL/AMoL cases and with a small number of AML cases (group-III: monocyte-“specific” reactivity). The MoAbs of group-I are useful in differentiating acute lymphoid from acute myeloid leukemias. The MoAbs of group-III, and to a lower extent those of group-II, will be of considerable value in the subtyping of acute myeloid leukemias. The results show that (1) accuracy of leukemia classification might not always be achieved by morphology alone, but that immunological and biochemical aspects should be included as well, and (2) several MoAbs are very useful tools for classification and subtyping of acute myeloid leukemias.
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References
Bennett JM, Catovsky D, Daniel M-Th, Flandrin G, Galton AG, Gralnick HR, Sultan C (1976) Proposals for the classification of the acute leukaemias. Br J Haematol 33:451
Bernard A, Boumsell L, Dausset J, Milstein C, Schlossman SF (1984) Leucocyte typing. Springer-Verlag, Berlin
Breard J, Reinherz EL, Kung PC, Goldstein G, Schlossman SF (1980) A monoclonal antibody reactive with human peripheral blood monocytes. J Immunol 124:1943
Civin CI, Mirro J, Banquerigo ML (1981) My-1, a new myeloid-specific antigen identified by a mouse monoclonal antibody. Blood 57:842
Dimitriu-Bona A, Burmester GR, Waters SJ, Winchester RJ (1983) Human mononuclear phagocyte differentiation antigens. I. Patterns of antigen expression on the surface of human monocytes and macrophages defined by monoclonal antibodies. J Immunol 130:145
Drexler HG, Gaedicke G (1983) Isoenzyme studies in human leukemia. II. Carboxylic esterase (E.C.3.1.1.1). Leuk Res 7:599
Drexler HG, Gaedicke G, Minowada J (1984) Enzyme markers in acute leukemias: Advances during the last decade. J Natl Cancer Inst 72:1283
Drexler HG, Sagawa K, Menon M, Minowada J (1985a) “Pan-myeloid” reagent: The monoclonal antibody MCS-2 in the routine immunodiagnostic service of leukemia phenotyping. Gann 76:235
Drexler HG, Gaedicke G, Minowada J (1985b) Isoenzyme studies in human leukemia-lymphoma cell lines. I. Carboxylic esterase. Leuk Res 9:209
Flandrin G, Daniel M-Th (1981) Cytochemistry in the classification of leukemias. In: Catovsky D (ed) The leukemic cell. Churchill-Livingstone, Edinburgh, p 29
Greaves MF, Grossi CE, Habeshaw JA, Lombardi L, Rilke F, Stansfeld AG (1981) Lymphocytes. In: Zucker-Franklin D, Greaves MF, Grossi CE, Marmont AM (eds) Atlas of blood cells — function and pathology. Lea & Febiger, Philadelphia, p 409
Griffin JD, Schlossman SF (1984a) Expression of myeloid differentiation antigens in acute myeloblastic leukemia. In: Bernard A, Boumsell L, Dausset J, Milstein C, Schlossman SF (eds) Leucocyte typing. Springer-Verlag, Berlin, p 404
Griffin JD, Ritz J, Nadler LM, Schlossman SF (1981) Expression of myeloid differentiation antigens on normal and malignant myeloid cells. J Clin Invest 68:932
Griffin JD, Linch D, Sabbath K, Larcom P, Schlossman SF (1984b) A monoclonal antibody reactive with normal and leukemic human myeloid progenitor cells. Leuk Res 8:521
Hanjan SNS, Kearney JF, Cooper MD (1982) A monoclonal antibody (MMA) that identifies a differentiation antigen on human myelomonocytic cells. Clin Immunol Immunopathol 23:172
Hoffbrand AV, Janossy G (1981) Enzyme and membrane markers in leukaemia: Recent developments. J Clin Pathol 34:254
Knapp W, Bettelheim P, Majdic O, Liszka K, Schmidmeier W, Lutz D (1984a) Diagnostic value of monoclonal antibodies to leucocyte differentiation antigens in lymphoid and non-lymphoid leukemias. In: Bernard A, Boumsell L, Dausset J, Milstein C, Schlossman SF (eds) Leucocyte typing. Springer-Verlag, Berlin, p 564
Knapp W, Majdic O, Bettelheim P, Liszka K, Aberer W, Stingl G (1984b) Typing of leukemic cells with monoclonal antibodies. Ann NY Acad Sci 420:251
Linch DC, Allen C, Beverley PCL, Bynoe AG, Scott CS, Hogg N (1984) Monoclonal antibodies differentiating between monocytic and nonmonocytic variants of AML. Blood 63:566
Majdic O, Liszka K, Lutz D, Knapp W (1981) Myeloid differentiation antigen defined by a monoclonal antibody. Blood 58:1127
Majdic O, Bettelheim P, Stockinger H, Aberer W, Liszka K, Lutz D, Knapp W (1984) M2, a novel myelomonocytic cell surface antigen and its distribution on leukemic cells. Int J Cancer 33:617
Minowada J (1985) Immunology of leukemia and lymphoma. In: Wiernik P (ed) Contemporary issues in clinical oncology, vol 4. Leukemias and lymphomas. Churchill-Livingstone, Edinburgh, p 183
Minowada J, Sagawa K, Trowbridge IS, Kung PC, Goldstein G (1982) Marker profiles of 55 human leukemia-lymphoma cell lines. In: Rosenberg SA, Kaplan HS (eds) Malignant lymphomas. Etiology, immunology, pathology, treatment. Academic Press, New York, p 53
Radzun HJ, Parwaresch M, Wittke JW (1981) Polymorphism and antigenic specificity of monocytic acid esterase (E.C.3.1.1.6). Cell Immunol 63:400
Reijden HJ van der, Rhenen DJ van, Lansdorp PM, Veer MB van't, Langenhuijsen MMAC, Engelfriet CP, Borne AEGKr von dem (1983) A comparison of surface marker analysis and FAB classification in acute myeloid leukemia. Blood 61:443
Scott CS, Linch DC, Bynoe AG, Allen C, Hogg N, Ainley MJ, Hough D, Roberts BE (1984) Electrophoretic and cytochemical characterization of alpha-naphthylacetate esterase in acute myeloid leukemia: Relationships with membrane receptor and monocyte-specific antigen expression. Blood 63:579
Todd III RF, Nadler LM, Schlossman SF (1981) Antigens on human monocytes identified by monoclonal antibodies. J Immunol 127:1435
Todd III RF, Bhan AK, Kabawat SE, Schlossman SF (1984) Human myelomonocytic differentiation antigens defined by monoclonal antibodies. In: Bernard A, Boumsell L, Dausset J, Milstein C, Schlossman SF (eds) Leucocyte typing. Springer-Verlag, Berlin, p 424
Tatsumi E, Sagawa K, Mirro J, Civin CI, Preisler HD, Henderson ES, Minowada J (1981) Immunologic membrane phenotypes in human myeloid leukemia by monoclonal antibodies. Proc Am Assoc Cancer Res 22:181
Ugolini V, Nunez G, Smith RG, Stastny P, Capra JD (1980) Initial characterization of monoclonal antibodies against human monocytes. Proc Natl Acad Sci USA 77:6764
Zittoun R, Cadiou M, Bayle C, Suciu S, Solbu G, Hayat M (1984) Prognostic value of cytologic parameters in acute myelogenous leukemia. Cancer 53:1526
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Drexler, H.G., Menon, M., Gaedicke, G. et al. Reactivity patterns of monoclonal antibodies positive on myelomonocytic leukemia cells as defined by esterase isoenzyme analysis. Cancer Immunol Immunother 21, 188–192 (1986). https://doi.org/10.1007/BF00199360
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DOI: https://doi.org/10.1007/BF00199360