Summary
Levels of secretory component (SC) were measured in breast tumors from 95 patients with primary or metastatic cancer. Tumor cytosols were prepared by polytron disruption and high speed centrifugation (105,000 g×30 min) and SC was measured using a sensitive radioimmunoassay which detects primarily free SC. In frozen samples stored for up to 5 months SC resisted degradation and could be measured quantitatively without interference. In primary tumors from patients between ages 28 and 97, SC positive samples ranged from 4 ng/mg protein to 600 ng/mg protein. In metastatic lesions, SC positive samples ranged no higher than 46 ng/mg protein. The studies indicate that SC can be measured quantitatively in both primary and metastatic tumors and that prolonged storage does not interfere with measurements of SC. The wide variation in SC levels in primary tumor samples may be related to a susceptibility to metastasis. Further, the low levels of SC in metastatic lesions could indicate a potential for SC involvement in immune regulation of tumor growth.
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Brandtzaeg P (1974) Mucosal and glandular distribution of immunoglobulin components: differential localization of free and bound SC in secretory epithelial cells. J Immunol 112:1553
Brandtzaeg P (1974) Human secretory component: Physiocochemical characterization of free secretory component purified from colostrum. Scand J Immunol 3:707
Crago SS, Kulhavy R, Prince SJ, Mestecky J (1984) Inhibition of the pokeweed mitogen-induced response of normal peripheral blood lymphocytes by humoral components of colostrum. Clin Exp Immunol 45:386
Draper N, Smith H (1981) Applied regression analysis. Wiley and Sons, New York
Dulbecco R, Vogt M (1954) Plaque formation and isolation of pure lines with poliomyelitis virus. J Exp Med 99:167
Epentos A, Mather S, Granowska M, Nimmon CC, Hawkins LR, Britton DE, Shepard J, Taylor-Papadimitriou J, Durbin H, Malpas JS, Bodmer WF (1982) Targeting of iodine-123-labelled tumour-associated monoclonal antibodies to ovarian, breast, and gastrointestinal tumours. Lancet II:999
Fialkow PJ (1974) The origin and development of human tumors studied with cell markers. N Engl J Med 291:26
Harris JP, Caleb MH, South MA (1975) Secretory component in human mammary carcinoma. Cancer Res 35:1861
Hsu S, Raine L, Nayak RN (1981) Medullary carcinoma of breast: an immunohistochemical study of its lymphoid stroma. Cancer 48:1368
Huggins C, Bergenstal DM (1952) Inhibition of human mammary and prostatic cancer by adrenalectomy. Cancer Res 12:134
Kelsey JL (1979) A review of the epidemiology of human breast cancer. Epidemiol Rev 1:74
Labib RS, Tomasi TB (1978) Secretory immunoglobulin A. CRC Handbook Clin Lab Sci F:137
Leung JP, Nelson-Rees WA, Moore GE, Calilleau R, Edgington TS (1981) Characteristics of membrane and cytosol forms of the mammary tumor glycoprotein molecule MTGP in human breast carcinoma cell cultures and tumors. Int J Cancer 28:35
Lowry OH, Rosenberg NJ, Farr AL, Randall RJ (1951) Protein measurements with the folin phenol reagent. J Biol Chem 193:265
Markwell MAK, Fox CF (1978) Surface-specific iodination of membrane proteins of viruses and eucaryotic cells using 1,3,4,6-tetrachloro-3-diphenylglycoluril. Biochemistry 17:4807
McGuire WL (1980) Steroid receptors and clinical breast cancer. In: Bresciani F (ed) Perspectives in steroid receptor research. Raven Press, New York, pp 239–246
McGuire WL (1980) Steroid receptors and breast cancer. Hosp Pract 15(4):83
McGuire WL, Horowitz KB, Chamness GC, Zava DT. (1976) A physiological role for estrogen and progesterone in breast cancer. J Steroid Biochem 7:875
Ogra SS, Ogra PL (1979) Components of immunologic reactivity in human colostrum and milk. In: Ogra PL, Dayton DH (eds) Immunology of breast milk. Raven Press, New York, pp 185–195
Puleo EAT Haagensen DE, Dawson JR, Gall SA (1979) Study of secretory component in patients with metastatic breast cancer. Am J Obstet Gynecol 134:899
Stern JE, Sullivan DA, Wira CR (1985) Secretory component in breast cancer: correlations with patient age and hormone receptor levels in primary breast tumors. Protides Biol Fluids 32:579
Sullivan DA, Allansmith MR (1984) Androgen regulation of IgA levels in tears of rats. Protides Biol Fluids 32:93
Sullivan DA, Wira CR (1983) Variations in free secretory component levels in mucosal secretions of the rat. J Immunol 130:1330
Sullivan DA, Wira CR (1981) Estradiol regulation of secretory component in the female reproductive tract. J Steroid Biochem 15:439
Sullivan DA, Underdown BJ, Wira CR (1983) Steroid hormone regulation of free secretory component in the rat uterus. Immunology 49:379
Sullivan DA, Richardson GS, MacLauglin DT, Wira CR (1984) Variations in the levels of secretory component in human uterine fluid during the menstrual cycle. J Steroid Biochem 20:509
Winzler RJ, Bocci V (1972) Turnover of plasma glycoproteins. In: Gottschalk A (ed), Glycoproteins. Elsevier, New York, pp 228–243
Wira CR, Sullivan DA, Sandoe CP (1983) Estrogen-mediated control of the secretory immune system in the uterus of the rat. Ann NY Acad Sci 409:534
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This work supported by research grant AI-13541 from NIH and CA-23108 from the NCI
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Stern, J.E., Underdown, B.J., Crichlow, R.W. et al. Secretory component in breast cancer. Cancer Immunol Immunother 19, 226–230 (1985). https://doi.org/10.1007/BF00199231
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DOI: https://doi.org/10.1007/BF00199231