Summary
The toxic agent purothionin was conjugated to the monoclonal antibody 225.28S to a human high-molecular-weight melanoma-accociated antigen. The toxic conjugate displayed in vitro toxicity to cultured human Colo 38 melanoma cells as indicated by reduced uptake of 3H-thymidine following a 24-h incubation and loss of cell viability following a 7-day incubation. The effect is dose-dependent and is specific since addition of the toxic conjugate to a cultured Raji B lymphoid cells did not affect their 3H-thymidine uptake or their viability.
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Bumol TF, Wang QC, Reisfeld RA, Kaplan NO (1983) Monoclonal antibody and an antibody-toxin conjugate to a cell surface proteoglycan of melanoma cells suppress in vivo tumor growth. Proc Natl Acad Sci USA 80:529
Burchiel SW, Martin JC, Imai K, Ferrone S, Warner NL (1982) Heterogeneity of HLA-A, B, Ia-like, and melanoma-associated antigen expression by human melanoma cell lines analysed with monoclonal antibodies and flow cytometry. Cancer Res 42:4110
Casellas P, Brown JP, Gros O, Gros P, Hellstrom I, Jansen FK, Poncelet P, Roncelet P, Roncucci R, Vidal H, Hellstrom KE (1982) Human melanoma cells can be killed in vitro by an immunotoxin specific for melanoma-associated antigen p97. Int J Cancer 30:437
Ghose T, Ferrone S, Imai K, Nowell ST, Luner SJ, Martin RH, Blair AH (1982) Imaging of human melanoma xenografts in nude mice with a radiolabelled monoclonal antibody. J Natl Cancer Inst 69:823
Gilliland GD, Steplewski Z, Collier RJ, Mitchell KF, Chang TH, Koprowski H (1980) Antibody-directed cytotoxic agents: Use of monoclonal antibody to direct the action of toxin A chains to colorectal carcinoma cells. Proc Natl Acad Sci USA 77:4539
Goodfriend TL, Levine L, Fasman GD (1964) Antibodies to bradykinin and angiotensin: A use of carbodiimides in immunology. Science 144:1344
Imai K, Ng AK, Ferrone S (1981a) Characterization of monoclonal antibodies to human melanoma-associated antigens. J Natl Cancer Inst 66:489
Imai K, Ng AK, Glassy MC, Ferrone S (1981b) ADCC of cultured human melanoma cells: Analysis with monoclonal antibodies to human melanoma-associated antigens. Scand J Immunol 14:369
Imai K, Nakanishi M, Matsui M, Noguchi T, Yachi A, Ferrone S (1983) In vivo effect of the conjugate of monoclonal antibody with cytotoxic agent on the tumor growth of human melanoma. Proc Biol Fluids 30:365
Krolick AK, Yuan D, Vitetta ES (1982) Specific killing of a human breast carcinoma cell line by a monoclonal antibody coupled to the A-chain or ricin. Cancer Immunol Immunother 12:39
Nakanishi R, Yoshizumi H, Takara S, Hakura A, Toyoshima K (1979) Cytotoxicity of purothionin-A on various animal cells. Gann 70:323
Natali PG, Imai K, Wilson BS, Bigotti A, Cabaliere R, Pellegrino MA, Ferrone S (1981) Structural properties and tissue distribution of the antigen recognized by the monoclonal antibody 653.40S to human melanoma cells. J Natl Cancer Inst 67:591
Natali PG, Cavaliere R, Bigotti A, Nicotra MR, Russo C, Ng AK, Giacomini P, Ferrone S (1983) Antigenic heterogeneity of surgically removed primary and antologous metastatic human melanoma lesions. J Immunol 130:1462
Ozaki A, Wada K, Hase T, Matsubara H, Nakanishi T, Yoshizumi H (1980) Amino acid sequence of a purothionin homolog from barley flour. J Biochem 87:549
Rowland GF, Simmonds RG, Corvalan JRF, Baldwin RW, Brown JP, Embleton MJ, Frod CHJ, Hellstrom KE, Hellstrom I, Kemshead JT, Newman CE, Woodhouse CS (1982) Monoclonal antibodies for targeted therapy with vindesine. Proc Biol Fluids 30:375
Sobol RE, Dillman RO, Smith JD, Imai K, Ferrone S, Shawler D, Glassy MC, Royston I (1982) Phase I evaluation of murine monoclonal anti-melanoma antibody in man: Preliminary observations. In: Hybridoma in cancer diagnosis and treatment. Raven Press, New York, p 199
Wilson BS, Imai K, Natali PG, Ferrone S (1981) Distribution and molecular characterization of a cell-surface and cytoplasmic antigen detectable in human melanoma cells with monoclonal antibodies. Int J Cancer 28:293
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Imai, K., Nakanishi, T., Noguchi, T. et al. Selective in vitro toxicity of purothionin conjugated to the monoclonal antibody 225.28S to a human high-molecular-weight melanoma-associated antigen. Cancer Immunol Immunother 15, 206–209 (1983). https://doi.org/10.1007/BF00199166
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DOI: https://doi.org/10.1007/BF00199166