Summary
C3HeB/FeJ spleen cells (unseparated or passaged over nylon wool columns) were cultured overnight (1∓2×106 cells/microwell) in the presence and absence of resident or ADM-induced PEC and anti-YAC-1 (4 h) NK activity was determined. The addition of resident PEC to spleen cells had little effect on NK activity. However, the addition of ADM-elicited PEC (10 mg/kg, IP, day −1 and day −5) to spleen cells prior to culture significantly augmented NK activity. If ADM-induced PEC were treated with carbonyl iron prior to coculture with spleen cells, augmentation of anti-YAC-1 activity was not observed. This suggested that ADM-activated macrophages augmented cultured splenic NK activity. Supernatants from overnight-cultured resident or ADM-induced adherent PEC were then prepared, dialyzed (to remove ADM), and tested for mitogenic activity or cocultured with spleen cells overnight. ADM-induced adherent PEC supernatants stimulated the proliferation of murine thymocytes (both LAF and IL-2 also stimulate) but not cultured CTL (only IL-2 stimulates). ADM-induced adherent PEC supernatants (as well as LAF, IL-2, and IFN) augmented overnight-cultured C3HeB/FeJ splenic NK activity. However, only IL-2 and IFN could augment overnight-cultured athymic BALB/c · nu/nu splenic NK activity. This suggested that ADM-elicited macrophages produce LAF which may act directly on NK cells or, more likely, may induce T cells to produce IL-2, IFN, or both.
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Abbreviations used in this paper: ADM, Adriamycin; NK, natural killer; PEC, peritoneal exudate cells; BCG, Bacillus Calmette-Guérin; LAF, lymphocyte-activating factor; IL-1, interleukin I; ppTCGF, partially; purified T cell growth factor; IL-2, interleukin 2; IFN, murine interferon; CTL, cytotoxic T lymphocytes; PMA, phorbal myristate acetate; ADM-PEC-Sup; adriamycin-elicited PEC supernatant; FCS, fetal calf serum; Indo, indomethacin; Ad, adherent
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Cohen, S.A., Salazar, D. & Wicher, J. Adriamycin-induced activation of NK activity may initially involve LAF production. Cancer Immunol Immunother 15, 188–193 (1983). https://doi.org/10.1007/BF00199163
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DOI: https://doi.org/10.1007/BF00199163