Abstract
The major light-harvesting complexes from Mantoniella squamata (Prasinophyceae) and from Chlorella fusca (Chlorophyceae) were analyzed with respect to polypeptide composition and pigmentation. It was found that the polypeptides of Mantoniella are smaller than those of Chlorella and bind twice the amount of pigment. We assume that the amount of pigment per polypeptide is of ecological as well as of taxonomical importance.
Abbreviations
- Chl:
-
chlorophyll
- LHC:
-
light-harvesting complex
- Xan:
-
xanthophyll
References
Bradford, M. (1976) A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248–254
Caron, L., Remy, R., Berkaloff, B. (1988) Polypeptide composition of light-harvesting complexes from some brown algae and diatoms. FEBS Lett. 229, 11–15
Fawley, M.W., Stewart, K.D., Mattox, K.R. (1986) The novel light-harvesting pigment-protein complex of Mantoniella squamata (Chlorophyta). J. Mol. Evol. 23, 168–176
Hiller, R., Larkum, A.W.D., Wrench, P. (1988) Chlorophyll-proteins of the prymnesiophyte Pavlova lutherii (Droop) comb, nov.: identification of the major light-harvesting complex. Biochim. Biophys. Acta 932, 223–231
Krämer, P., Wilhelm, C., Wild, A., Mörschel, E., Rhiel, E. (1988) Ultrastructure and freeze fracture studies of the thylakoids of Mantoniella squamata (Prasinophyceae). Protoplasma 147, 170–177
Laemmli, U. (1970) Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature 227, 680–685
Larkum, A., Hiller, R. (1988) Light-harvesting complexes of chlorophyll c-containing algae. In: Photosynthetic light-harvesting systems, pp. 153–165, Scheer, H., Schneider, S., eds. de Gruyter, Berlin
Lowry, O.K., Rosebrough, N.J., Farr, A.L., Randall, R.J. (1951) Protein measurement with a Folin phenol reagent. J. Biol. Chem. 193, 265–275
Nakayama, K., Itagaki, T., Okada, M. (1986) Pigment composition of chlorophyll-protein complexes isolated from the green alga Bryopsis maxima. Plant Cell Physiol. 27, 311–317
Round, F.E. (1984) The systematics of the Chlorophyta: an historical review leading to some modern concepts. In: The systematics of the green algae, pp. 1–28, Irvine, D.E.G., John, D.M., eds. Academic Press, London
Siefermann-Harms, D. (1985) Carotenoids in photosynthesis: location in photosynthetic membranes and light-harvesting function. Biochim. Biophys. Acta 811, 325–355
Thornber, J.P., Peter, G.F., Chitnis, P.R., Nechustai, R., Vainstein, A. (1988) The light-harvesting complex of photosystem II of higher plants. In: Light-energy transduction in photosynthesis, pp. 137–154, Stevens, S.E., Bryant, D.A., eds. Alan Liss, New York
Wilhelm, C. (1989) The biochemistry and physiology of light harvesting processes in chlorophyll b- and chlorophyll c-containing algae. Plant Physiol. Biochem. 28, (2)
Wilhelm, C., Lenartz-Weiler, I. (1987) Energy transfer and pigment composition in three chlorophyll b-containing light-harvesting complexes isolated from Mantoniella squamata (Prasinophyceae), Chlorella fusca (Chlorophyceae) and Sinapis alba. Photosynth. Res. 13, 125–141
Wilhelm, C., Wild, A. (1980) The effect of vanadium on the content of chlorophyll, P-700 and cytochrome f at different light intensities in Chlorella fusca. Biochem. Physiol. Pflanz. 175, 163–171
Wilhelm, C., Lenartz-Weiler, I., Wiedemann, I., Wild, A. (1986) The light-harvesting system of Micromonas spec., Butcher (Prasinophyceae): the combination of three different chlorophyll species in one single chlorophyll-protein complex. Phycologia 25, 304–312
Wilhelm, C., Büchel, C., Rousseau, B. (1988) The molecular organization of chlorophyll-protein complexes in the xanthophycean alga Pleurochloris meiringensis. Biochim. Biophys. Acta 934, 220–226
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We thank the support by the Deutsche Forschungsgemeinschaft.
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Wilhelm, C., Wiedemann, I. & May, M. Comparative analysis of the composition of two chlorophyll-b-containing light-harvesting complexes. Planta 180, 456–457 (1990). https://doi.org/10.1007/BF00198800
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DOI: https://doi.org/10.1007/BF00198800