Skip to main content
Log in

Restriction site mapping of adenovirus prototypes of subgenus D

  • Original Investigations
  • Published:
Medical Microbiology and Immunology Aims and scope Submit manuscript

Abstract

Restriction site maps with the endonucleases HindIII, BglII, and Bam HI were elaborated for the majority of 23 adenovirus prototypes of subgenus D (AV8 to AV39). On the basis of four or five types which have been mapped by biochemical methods, the physical maps for other types were constructed by adapting restriction fragments of defined molecular weight, as the homology among types of the same subgenus is considerable. Prototypes of subgenus D shared several common restriction sites; different sites, however, were distributed at random over the genome. The gene loci for hexon and fiber did not show either higher or lower variability.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Adrian T, Wigand R (1989) Genome type analysis of certain serotypes of subgenus D. Zentralbl Bakteriol Mikrobiol Hyg [A] 270:527–533

    Google Scholar 

  2. Adrian T, Wadell G, Hierholzer JC, Wigand R (1986) DNA restriction analysis of adenovirus prototypes 1 to 41. Arch Virol 91:277–290

    Google Scholar 

  3. Adrian T, Bastian B, Wagner V (1989) Restriction site mapping of adenovirus types 9 and 15 and genome types of intermediate adenovirus 15/H9. Intervirology 30:169–176

    Google Scholar 

  4. Broker TR (1980) Nucleotide sequence and analysis of group C adenoviruses. In: Tooze J (ed) DNA tumor viruses. Molecular biology of tumor viruses, 2nd edn, part 2. Cold Spring Harbor Laboratory, Cold Spring Harbor, pp 923–935

    Google Scholar 

  5. Duggleby RG, Kinns H, Rood JI (1981) A computer program for determining the size of DNA restriction fragments. Anal Biochem 110:49–55

    Google Scholar 

  6. Gomes SA, Niel C (1987) Human adenoviruses from subgenus D: restriction mapping of types 9 and 19 and characterization of a new genome type. J Gen Virol 68:3173–3175

    Google Scholar 

  7. Green M, Mackey JK, Wold WSM, Rigden P (1979) Thirty-one human adenovirus serotypes (Ad1–31) form five groups (A-E) based upon DNA genome homologies. Virology 93:481–492

    Google Scholar 

  8. Hierholzer JC, Wigand R, Anderson LJ, Adrian T, Gold JWM (1988) Adenoviruses from AIDS patients: plethora of serotypes and description of five new serotypes of subgenus D (types 43–47). J infect Dis 158:804–813

    Google Scholar 

  9. Hierholzer JC, Wigand R, de Jong JC (1988) Evaluation of human adenoviruses 38, 39, 40 and 41 as new serotypes. Intervirology 29:1–10

    Google Scholar 

  10. Schmitz H, Wigand R, Heinrich W (1983) Worldwide epidemiology of human adenovirus infections. Am J Epidemiol 117:455–466

    Google Scholar 

  11. Takacs M, Berencsi G, Lengyel A, Nasz I (1983) Restriction site maps of the human adenovirus type 8 DNA. Acta Virol 27:289–298

    Google Scholar 

  12. Wadell G, de Jong JC (1980) Restriction endonucleases in identification of a genome type of adenovirus 19 associated with keratoconjunctivitis. Infect Immun 27:292–296

    Google Scholar 

  13. Wigand R, Adrian T (1989) Intermediate adenovirus strains of subgenus D occur in extensive variety. Med Microbiol Immunol 178:37–44

    Google Scholar 

  14. Wigand R, Bartha A, Dreizin RS, Esche H, Ginsberg HS, Green M, Hierholzer JC, Kalter SS, McFerran JB, Pettersson U, Russell WC, Wadell G (1982) Adenoviridae: second report. Intervirology 18:169–176

    Google Scholar 

  15. Wigand R, Adrian T, Bricout F (1987) A new human adenovirus of subgenus D: candidate adenovirus type 42. Arch Virol 94:283–288

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wigand, R., Adrian, T. Restriction site mapping of adenovirus prototypes of subgenus D. Med Microbiol Immunol 179, 67–75 (1990). https://doi.org/10.1007/BF00198527

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00198527

Keywords

Navigation