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Resolution and sensitivity enhancement of heteronuclear correlation for methylene resonances via 2H enrichment and decoupling

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Summary

Monodeuterated methylene positions exhibit substantially superior spectral characteristics in 1H−13C correlation experiments as compared to diprotio signals. A combination of 2H decoupling and multiplet editing of HMQC and HSQC experiments provides resolution enhancement for both stereoselective and random fractionally deuterated samples. For HMQC experiments with [2-2Hr, 2-13C]glycine-labeled E. coli thioredoxin (11.7 kDa), 3-fold increases in both 1H and 13C resolution result in a complementary 9-fold enhancement in sensitivity. Owing to a smaller improvement in 13C resolution, the corresponding enhancements for the HSQC experiment are 2-fold less.

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Authors and Affiliations

  1. Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, 60208, Evanston, IL, USA

    Diana M. Kushlan & David M. LeMaster

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  1. Diana M. Kushlan
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  2. David M. LeMaster
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Kushlan, D.M., LeMaster, D.M. Resolution and sensitivity enhancement of heteronuclear correlation for methylene resonances via 2H enrichment and decoupling. J Biomol NMR 3, 701–708 (1993). https://doi.org/10.1007/BF00198372

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  • DOI: https://doi.org/10.1007/BF00198372

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