Summary
A new pulse sequence is presented allowing the use of long selective pulses at the water frequency using standard equipment. Radiation damping is suppressed during the pulse by the use of gradient echoes programmed between the single pulses of a DANTE train. This WANTED (water-selective DANTE using gradients) sequence thus allows the observation of interactions with water without the use of special probe heads or filtering of undesired resonances. By combining the WANTED sequence with NOESY, ROESY and NOESY-GSQC experiments, we obtain selective 1D and 2D spectra fit to the observation of chemical exchange and dipolar interactions between water and protein protons.
This is a preview of subscription content, log in via an institution to check access.
References
BloembergenN. and PoundR.V. (1954) Phys. Rev., 95, 8–12.
BloomS. (1957) J. Appl. Phys., 28, 800–805.
BöckmannA., PeninF. and GuittetE. (1996) FEBS Lett., 383, 191–195.
BroekaertP. and JeenerJ. (1995) J. Magn. Reson., A113, 60–64.
DalvitC. (1995) J. Magn. Reson., A113, 120–123.
DalvitC. and HommelU. (1995) J. Magn. Reson., B109, 334–338.
DelsucM.A. (1989) In Maximum Entropy and Bayesian Methods, (Ed. SkillingJ.), Kluwer, Dordrecht, The Netherlands.
GemmeckerG., JahnkeW. and KesslerH. (1993) J. Am. Chem. Soc., 115, 11620–11621.
GrzesiekS. and BaxA. (1993a) J. Am. Chem. Soc., 115, 12593–12594.
GrzesiekS. and BaxA. (1993b) J. Biomol. NMR, 3, 627–638.
KriwackiR.W., HillR.B., FlanaganJ.M., CaradonnaJ.P. and PrestegardJ.H. (1993) J. Am. Chem. Soc., 115, 8907–8911.
Louis-JosephA., AbergelD. and LallemandJ.-Y. (1995) J. Biomol. NMR, 5, 212–216.
MoriS., BergJ.M. and VanZijlP.C.M. (1996) J. Biomol. NMR, 7, 77–82.
MorrisG.A. and FreemanR. (1978) J. Magn. Reson., 29, 433–462.
OttingG. and LiepinshE. (1995a) J. Magn. Reson., B107, 192–196.
OttingG. and LiepinshE. (1995b) J. Biomol. NMR, 5, 420–426.
Penin, F., Geourjon, C., Montserret, R., Yang, Y., Fillon, A., Bonod, C., Cortay, J.C., Nègre, D., Cozzone, A.J. and Deléage, G., manuscript submitted for publication.
PicardL., v. KienlinM., DécorpsM. (1995) J. Magn. Reson., A117, 262–266.
PiottoM., SaudekV. and SklenářV. (1992) J. Biomol. NMR, 2, 661–665.
ScarabelM., PeninF., BonodC., NègreD., CozzoneA.J. and CortayJ.C. (1995) Gene, 153, 9–15.
ShakaA.J., BarkerP.B. and FreemanR. (1985) J. Magn. Reson., 64, 547–552.
SklenářV. (1995) J. Magn. Reson., A114, 132–135.
ZhangS. and GorensteinD.G. (1996) J. Magn. Reson., A118, 291–294.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Böckmann, A., Guittet, E. Suppression of radiation damping during selective excitation of the water signal: The WANTED sequence. J Biomol NMR 8, 87–92 (1996). https://doi.org/10.1007/BF00198142
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00198142